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Method for inducing DC-CIK by utilizing muramyl dipeptide

A technology of DC-CIK and MDP-DC-CIK, applied in the field of cell wall dipeptide, can solve the problem of high activity of killing tumor cells, achieve the effect of improving tumor killing activity, improving the quality of life, and promoting T cell differentiation

Active Publication Date: 2014-04-09
中海峡福建细胞生物科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0031] However, the current conventionally induced and cultured DC-CIK cells are not very active in killing tumor cells, which requires a new induction method

Method used

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  • Method for inducing DC-CIK by utilizing muramyl dipeptide
  • Method for inducing DC-CIK by utilizing muramyl dipeptide
  • Method for inducing DC-CIK by utilizing muramyl dipeptide

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Embodiment Construction

[0051] The preferred embodiments of the present invention will be described in detail below.

[0052] 1. Muramyl dipeptide (MDP) synthesis

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Abstract

The invention provides a method for inducing DC-CIK by utilizing muramyl dipeptide, that is to say, the muramyl dipeptide is added into a CIK or DC-CIK culture solution to induce proliferation and differentiation of CIK or DC-CIK cells, so as to improve the tumor-killing activity of the CIK or DC-CIK cells. The method comprises the following steps: peripheral blood collection, tumor antigen acquisition, mononuclear cell separation, mononuclear cell collection, mononuclear cell washing, MDP-DC-CIK cell induction, and culture. MDP-DC-CIK cells introduced and cultured by the method are detected by a flow cytometry, the ratio of CD3+CD56+ non-MHC restricted NKT cells is found to be up to 80% or more; and at the same time, the activity of tumor killer cells is much higher than that of DC-CIK cells by conventional induction culture, and a laboratory test result of the tumor-killing percentage reaches 99% or more.

Description

Technical field [0001] The present invention relates to the field of immune technology, in particular to a cell wall dipeptide obtained by using protein peptide synthesis technology, which is added to an induction medium to induce the proliferation and differentiation of MDP-DC-CIK cells, so that the tumoricidal activity of MDP-DC-CIK is greatly improved Methods. Background technique [0002] Tumor cell biotherapy is an emerging tumor treatment model with significant curative effect, and a new type of treatment method for autoimmune cells to fight cancer. It is a method that uses biotechnology and preparations to induce culture and amplify the immune cells collected from tumor patients in vitro, and then return them to the patient's body. By stimulating, regulating and enhancing the body's own immune function, it can achieve the purpose of treating tumors. . Tumor biotherapy is another tumor treatment technology after surgery, radiotherapy and chemotherapy, and it is called the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784C12N5/0783A61K35/14A61P35/00A61K35/15
Inventor 吴炯张炬
Owner 中海峡福建细胞生物科技有限公司
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