Anti-duck virus hepatitis (DVH) subprostrate sophora polysaccharide and molecular modifier thereof
A technology for duck viral hepatitis and duck hepatitis virus, which is applied in the directions of antiviral agents, organic active ingredients, medical preparations containing active ingredients, etc. good effect
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[0016] 1. Extraction of polysaccharides from bean root and screening of effective fractions
[0017] Weigh 1,000g of the bean root, add 10 times the amount of water to soak for 3 hours, heat and boil for 1 hour, collect the decoction, and repeat 3 times; combine the collected decoction, boil and concentrate to 1000mL, centrifuge with a high-speed centrifuge, and take the supernatant. Slowly add ethanol with different volume fractions of 95% to the concentrated solution so that the ethanol content in the solution is 50%, 65%, 75% and 80% respectively, let it stand overnight, and collect the precipitates by centrifugation respectively. After dialysis with running water, vacuum concentration, 3000rpm / min, and centrifugation for 20min, the supernatant was again added with different volume fractions of 95% ethanol to precipitate, after repeated dialysis, concentration, and centrifugation, vacuum-dried at 60°C, and crushed to obtain different polysaccharides of bean root. Alcohol pr...
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