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Anti-duck virus hepatitis (DVH) subprostrate sophora polysaccharide and molecular modifier thereof

A technology for duck viral hepatitis and duck hepatitis virus, which is applied in the directions of antiviral agents, organic active ingredients, medical preparations containing active ingredients, etc. good effect

Active Publication Date: 2014-04-23
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Once a clinical case occurs, it will cause irreparable losses

Method used

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  • Anti-duck virus hepatitis (DVH) subprostrate sophora polysaccharide and molecular modifier thereof
  • Anti-duck virus hepatitis (DVH) subprostrate sophora polysaccharide and molecular modifier thereof
  • Anti-duck virus hepatitis (DVH) subprostrate sophora polysaccharide and molecular modifier thereof

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Embodiment Construction

[0016] 1. Extraction of polysaccharides from bean root and screening of effective fractions

[0017] Weigh 1,000g of the bean root, add 10 times the amount of water to soak for 3 hours, heat and boil for 1 hour, collect the decoction, and repeat 3 times; combine the collected decoction, boil and concentrate to 1000mL, centrifuge with a high-speed centrifuge, and take the supernatant. Slowly add ethanol with different volume fractions of 95% to the concentrated solution so that the ethanol content in the solution is 50%, 65%, 75% and 80% respectively, let it stand overnight, and collect the precipitates by centrifugation respectively. After dialysis with running water, vacuum concentration, 3000rpm / min, and centrifugation for 20min, the supernatant was again added with different volume fractions of 95% ethanol to precipitate, after repeated dialysis, concentration, and centrifugation, vacuum-dried at 60°C, and crushed to obtain different polysaccharides of bean root. Alcohol pr...

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Abstract

The invention relates to anti-duck virus hepatitis (DVH) subprostrate sophora polysaccharide and a molecular modifier thereof, belonging to the technical field of preparation of Chinese veterinary medicaments. Different alcohol precipitation fractions of the subprostrate sophora polysaccharide are extracted. An anti-duck virus hepatitis (DVH) active site is screened by means of repeated clinical tests. The following optimal conditions for modifying a subprostrate sophora polysaccharide active site by means of chlorosulfonic acid-pyridine sulfation are determined by using an orthogonal method: the reaction temperature is 95 DEG C, the reaction time is 2 hours, and the ratio of chlorosulfonic acid to pyridine is 1:5. According to infrared spectroscopic analysis, an obtained product is subprostrate sophora polysaccharide sulfate, the saccharide content is up to 83.77 percent, the sulfate content is up to 39.33 percent, and the yield is up to 117.33 percent. The subprostrate sophora polysaccharide sulfate is remarkably superior to the subprostrate sophora polysaccharide in resistance of DHV-1 infection of duck fetal liver cells and curative effect on DVH. The obtained anti-DVH subprostrate sophora polysaccharide active site sulfate is high in saccharide and phosphate contents and yield, and has a good curative effect on DVH.

Description

1. Technical field [0001] The invention relates to an anti-duck viral hepatitis polysaccharide and its molecular modification, and belongs to the technical field of Chinese veterinary medicine preparation. 2. Background technology [0002] Duck viral hepatitis is a disease caused by duck hepatitis virus (DHV) infection that spreads rapidly, has a high morbidity rate, and is highly fatal. The virus was first isolated in 1949 and is now distributed worldwide. DHV mainly has three serotypes, DHV-1 and its mutant strains, Duck Astrovirus and DHV-3, among which DHV-1 is the most toxic and widely distributed, and mainly infects ducklings under 3 weeks old, with a fatality rate higher than 80%, even up to 100%, is one of the main pathogens that seriously endanger the duck industry. [0003] At present, there is no effective anti-DHV-1 drug clinically in the world, and the immune defense is mainly carried out by injecting attenuated vaccines to breeding ducks or ducklings. Once a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/14
Inventor 刘家国陈云熊文曾玲徐美云王德云胡元亮武毅黄兵马秀丽
Owner NANJING AGRICULTURAL UNIVERSITY
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