Optimizing Process of Environmental Conditions for Liquid Fermentation of I. fumigatus scau-ifcf02 Strain
A technology of I. fumigatus and liquid fermentation, which is applied in the direction of using spores, biochemical equipment and methods, microorganisms, etc., and can solve the problems that affect the production of biological preparations related to I. fumigatus and restrict the production of I. fumigatus Problems such as popularization and application of Bacillus spp., vulnerable to external unfavorable conditions, etc., to achieve good control ability, short cycle, and less number of tests
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Embodiment 1
[0042] The preparation of the spore suspension of embodiment 1 rose fume-colored clavula SCAU-IFCF02 strain
[0043] The preparation method of the spore suspension of I. fumigatus SCAU-IFCF02 bacterial strain is as follows:
[0044] S1. Take I. fumigatus SCAU-IFCF02 strain, under sterile conditions, use an inoculation loop to take a loop and inoculate it on a potato dextrose agar medium (PDA) plate for activation. ) in a constant temperature incubator for 15 days;
[0045] S2. After the mycelia cover the culture dish, add 20 mL of sterile water containing 0.03% Tween-80, scrape the fungal mycelia and spores lightly with an inoculation needle, pour the bacterial liquid into a beaker, and stir with a magnetic stirrer 30min;
[0046] S3. After the spores are completely dispersed, filter the bacterial liquid with double-layer medical gauze, discard impurities and culture medium residues, and obtain a conidia suspension;
[0047] S4. Count the spore concentration of the spore ...
Embodiment 2
[0049] The influence of embodiment 2 cultivation temperature on mycelia biomass and spore production of I. fumigatus I. fumosae SCAU-IFCF02 strain
[0050] 1. Test settings
[0051] (1) The spore suspension of the strain I. fumigatus rosea SCAU-IFCF02 prepared in Example 1 was used as the seed liquid for fermentation. The components of the fermentation medium were glucose 39g / L and yeast extract 19g / L. The sample volume was 75mL / 250mL, and the inoculum volume of I. fumigatus SCAU-IFCF02 strain was 5% (V / V).
[0052] (2) During the shaker culture process, follow the principle of single factor variable. The initial pH value is the natural pH value of the culture solution, and the shaker speed is 150 rpm. The temperature conditions were set at 19°C, 22°C, 25°C, 28°C and 31°C, and the effects of different culture temperatures on mycelia biomass and sporulation were determined, and the shaker was cultured for 6 days. The mycelial biomass and sporulation were measured every 24...
Embodiment 3
[0054] Example 3 Effect of initial pH value on mycelial biomass and sporulation of I. fumigatus rosea SCAU-IFCF02 strain
[0055] 1. Test settings
[0056] (1) The spore suspension of the strain I. fumigatus rosea SCAU-IFCF02 prepared in Example 1 was used as the seed liquid for fermentation. The components of the fermentation medium were glucose 39g / L and yeast extract 19g / L. The sample volume was 75mL / 250mL, and the inoculum volume of I. fumigatus SCAU-IFCF02 strain was 5% (V / V).
[0057] (2) On the basis of the selected optimal culture temperature for sporulation, control the rotation speed of the shaker to 150rpm. The initial pH value of the culture solution is set as follows: 4, 5, 6, 7, 8 and 9, the initial pH value is allocated by NaOH and HCl dilution, and the influence of different initial pH values on mycelia biomass and sporulation is measured. Other conditions and determination methods are the same as those described above, and the optimum initial pH value f...
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