a cell preservation solution
A preservation solution and cell technology, applied in the field of cell preservation solution, can solve the problems of rapid decline in cell activity and high cost, and achieve the effect of long validity period, low cost, maintenance of cell viability and stem cell proliferation activity
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Embodiment 1
[0032] Embodiment 1 uses the preparation of cell preservation solution of the present invention
[0033] Take 7.020g of NaCl, 0.164g of KCl, 0.774g of sodium citrate, 2.700g of glucose, 20.066g of CaCl, 15.000g of albumin, 3.000g of amino acids, and 0.150g of vitamins, and dissolve them in 1000ml of water;
[0034] Made of NaCl120mM, KCl2.2mM, sodium citrate 3mM, glucose 15mM, CaCl 2 The solution of 0.6mM, 1.5% (w / v) albumin, 0.3% (w / v) amino acid and 0.015% (w / v) vitamin is the cell preservation solution of the present invention.
[0035] After testing, the pH of the preservation solution is in the range of 7.0-7.4, and the osmotic pressure is in the range of 280-320mOsm / L.
Embodiment 2
[0036] Embodiment 2 uses the preparation of cell preservation solution of the present invention
[0037] Take 7.020g of NaCl, 0.164g of KCl, 0.774g of sodium citrate, 2.700g of glucose, 2.730g of mannitol, 20.066g of CaCl, 15.000g of albumin, 3.000g of amino acids, and 0.150g of vitamins, and dissolve them in 1000ml of water;
[0038] Made of NaCl120mM, KCl2.2mM, sodium citrate 3mM, glucose 15mM, mannitol 15mM, CaCl 2 The solution of 0.6mM, 1.5% (w / v) albumin, 0.3% (w / v) amino acid and 0.015% (w / v) vitamin is the cell preservation solution of the present invention.
[0039] After testing, the pH of the preservation solution is in the range of 7.0-7.4, and the osmotic pressure is in the range of 280-320mOsm / L.
Embodiment 3
[0040] Embodiment 3 uses the preparation of cell preservation solution of the present invention
[0041] Take 8.014g of NaCl, 0.209g of KCl, 2.322g of sodium citrate, 3.600g of glucose, 0.133g of CaCl, 35.000g of albumin, 8.000g of amino acids, and 0.350g of vitamins, and dissolve them in 1000ml of water;
[0042] Made of NaCl137mM, KCl2.8mM, sodium citrate 9mM, glucose 20mM, CaCl 2 The solution of 1.2mM, 3.5% (w / v) albumin, 0.8% (w / v) amino acid and 0.035% (w / v) vitamin is the cell preservation solution of the present invention.
[0043] After testing, the pH of the preservation solution is in the range of 7.0-7.4, and the osmotic pressure is in the range of 280-320mOsm / L.
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