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a cell preservation solution

A preservation solution and cell technology, applied in the field of cell preservation solution, can solve the problems of rapid decline in cell activity and high cost, and achieve the effect of long validity period, low cost, maintenance of cell viability and stem cell proliferation activity

Active Publication Date: 2015-11-18
SICHUAN NEO LIFE STEM CELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Normal saline is a commonly used infusion medium, which is convenient and safe in clinical application, but the activity of cells in normal saline decreases very quickly, and the loss of cell activity can reach as much as 30% within 10 hours, so it is only suitable for short-term storage of cells , immediate infusion
Plasma can maintain cell activity well, but plasma contains a large number of unknown components, allogeneic infusion has potential safety hazards, and homologous infusion is extremely costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 uses the preparation of cell preservation solution of the present invention

[0033] Take 7.020g of NaCl, 0.164g of KCl, 0.774g of sodium citrate, 2.700g of glucose, 20.066g of CaCl, 15.000g of albumin, 3.000g of amino acids, and 0.150g of vitamins, and dissolve them in 1000ml of water;

[0034] Made of NaCl120mM, KCl2.2mM, sodium citrate 3mM, glucose 15mM, CaCl 2 The solution of 0.6mM, 1.5% (w / v) albumin, 0.3% (w / v) amino acid and 0.015% (w / v) vitamin is the cell preservation solution of the present invention.

[0035] After testing, the pH of the preservation solution is in the range of 7.0-7.4, and the osmotic pressure is in the range of 280-320mOsm / L.

Embodiment 2

[0036] Embodiment 2 uses the preparation of cell preservation solution of the present invention

[0037] Take 7.020g of NaCl, 0.164g of KCl, 0.774g of sodium citrate, 2.700g of glucose, 2.730g of mannitol, 20.066g of CaCl, 15.000g of albumin, 3.000g of amino acids, and 0.150g of vitamins, and dissolve them in 1000ml of water;

[0038] Made of NaCl120mM, KCl2.2mM, sodium citrate 3mM, glucose 15mM, mannitol 15mM, CaCl 2 The solution of 0.6mM, 1.5% (w / v) albumin, 0.3% (w / v) amino acid and 0.015% (w / v) vitamin is the cell preservation solution of the present invention.

[0039] After testing, the pH of the preservation solution is in the range of 7.0-7.4, and the osmotic pressure is in the range of 280-320mOsm / L.

Embodiment 3

[0040] Embodiment 3 uses the preparation of cell preservation solution of the present invention

[0041] Take 8.014g of NaCl, 0.209g of KCl, 2.322g of sodium citrate, 3.600g of glucose, 0.133g of CaCl, 35.000g of albumin, 8.000g of amino acids, and 0.350g of vitamins, and dissolve them in 1000ml of water;

[0042] Made of NaCl137mM, KCl2.8mM, sodium citrate 9mM, glucose 20mM, CaCl 2 The solution of 1.2mM, 3.5% (w / v) albumin, 0.8% (w / v) amino acid and 0.035% (w / v) vitamin is the cell preservation solution of the present invention.

[0043] After testing, the pH of the preservation solution is in the range of 7.0-7.4, and the osmotic pressure is in the range of 280-320mOsm / L.

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Abstract

The invention discloses a cell preserving liquid. The cell preserving liquid contains 120-137mM of NaCl, 2.2-2.8mM of KCl, 3-9mM of sodium citrate, 15-20mM of glucose, 0.6-1.2mM of CaCl2, 1.5-3.5%(w / v) of alhumin, 0.3-0.8%(w / v) of amino acids and 0.015-0.035%(w / v) of vitamins. The invention also discloses a preparation method of the cell preserving liquid, and a cell preserving method. The cell preserving liquid is safe, can maintain the motility rate of cells for a long time, has a low cost, and has a good application prospect and a good economic benefit.

Description

technical field [0001] The invention relates to a cell preservation solution. Background technique [0002] Cell, the most basic unit of life, has application value in many aspects. Stem cells (stem cells, SC) are a kind of pluripotent cells with self-renewing ability (self-renewing), under certain conditions, it can differentiate into a variety of functional cells. Stem cells (StemCell) are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. Mesenchymal stem cells (mesenchymal stem cells, MSC) are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Originally found in bone marrow, MSCs have attracted increasing attention because of their multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replicati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/00A01N1/02
Inventor 陈强罗茂文赖真阳钟立武
Owner SICHUAN NEO LIFE STEM CELL BIOTECH
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