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Measuring method of soluble saccharide

A determination method and solubility technology, which are applied in the preparation of test samples, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of long time consumption and interference in chlorophyll determination. , prone to turbidity and other problems, to achieve the effect of reducing time and cost, reducing sampling quality, and reducing experimental errors

Inactive Publication Date: 2014-06-04
JILIN UNIV
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Problems solved by technology

[0006] There are also certain disadvantages in the determination method of anthrone sulfuric acid colorimetry, such as cumbersome operation steps and long time consumption; chlorophyll interferes with the determination; when anthrone reagent is prepared, due to Anthrone molecules are relatively active, and both light and heat can easily change its molecular structure. Insufficient cooling after sulfuric acid preparation or delay in preparation of reagents will affect the quality of anthrone reagents, and yellow-green is prone to appear; It will affect the colorimetric effect; if the soluble sugar content is high, the volume of anthrone will be small, the reaction will not be sufficient, and turbidity will easily appear

Method used

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  • Measuring method of soluble saccharide
  • Measuring method of soluble saccharide
  • Measuring method of soluble saccharide

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Embodiment Construction

[0040] see figure 2 Shown, the assay method of soluble sugar of the present invention comprises the following steps:

[0041] Step a1. Sample pretreatment:

[0042] The plant tissues (such as leaves) are killed at 110°C (or in liquid nitrogen) for 15 minutes, dried at 70°C, crushed, and passed through a 40-mesh sieve;

[0043] Step a2. Distilled water extracts soluble sugar:

[0044] Weigh 20 mg of the sample into a 10 mL graduated test tube with a stopper, add 10 mL of distilled water to the graduated test tube, cover the stopper, and place it on an aluminum test tube rack; place it in a water bath at 80°C for 60 minutes, and add 40 mg of it to it Activated carbon, cover the stopper, shake and shake repeatedly; decolorize in the 80°C water bath for 30 minutes, take it out and cool to room temperature (place the aluminum test tube rack in a basin filled with tap water), filter and take the filtrate for determination.

[0045] Step a3. Color development and color comparison...

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Abstract

The invention relates to a measuring method of a soluble saccharide. The method includes specific steps of: pretreating a sample, extracting the soluble saccharide with distilled water, developing and comparing color, making a standard curve, and calculating the content of the soluble saccharide. According to the method, different sample pretreating methods are selected according to different experiment conditions and measuring contents, so as to guarantee conformity of experiment conditions. Experiment steps are simplified, namely, the sampling weight is reduced and steps of centrifuging, collecting liquid supernatant, combining the liquid supernatant, achieving a constant volume, and the like are omitted. Exploration of extraction is performed in a scale test tube with a stopper, thus reducing the time and the cost. The method is particularly suitable for measurement of samples in large batches.

Description

technical field [0001] The invention relates to a method for measuring soluble sugar, in particular to a fast and economical method for measuring soluble sugar. Background technique [0002] The measuring method of soluble sugar in the prior art generally uses glucose as the measuring index of soluble sugar, and measuring method has 4 kinds, and reagent reaction is violent during phenol sulfuric acid method measuring, and liquid splashes easily, and phenol is easily oxidized; Fehling's reagent colorimetric method Methylene blue will be oxidized in contact with air; 3, 5-dinitrosalicylic acid colorimetric method is only for the determination of reducing sugar; anthrone sulfuric acid colorimetric method has stable color development, good reproducibility, and is easy to operate. It is relatively stable and the result is accurate and reliable. The principle is that soluble sugar generates furfural or hydroxymethylfurfural compound under the action of sulfuric acid, and the latte...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N21/78
Inventor 丁雪梅
Owner JILIN UNIV
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