Bactericide and process for producing agricultural amino acid fertilizer by detoxifying and hydrolyzing rape seed cake

A technology of rapeseed cake and amino acid, which is applied in the preparation of organic fertilizers, methods based on microorganisms, applications, etc., can solve the problems of difficult industrial utilization, inability to completely remove toxic substances, etc., and achieve health protection, growth promotion, and protection The effect of ecological environment

Active Publication Date: 2014-06-25
XINGHUA NEW SOIL SOURCE BASE FERTILIZER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical detoxification has certain effects, but it cannot completely remove toxic substances, and there are still certain difficulties in industrial application

Method used

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  • Bactericide and process for producing agricultural amino acid fertilizer by detoxifying and hydrolyzing rape seed cake
  • Bactericide and process for producing agricultural amino acid fertilizer by detoxifying and hydrolyzing rape seed cake
  • Bactericide and process for producing agricultural amino acid fertilizer by detoxifying and hydrolyzing rape seed cake

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 strain screening

[0029] 1. Obtaining rapeseed cake

[0030] Provided by Jiangsu Xintiandi Biological Fertilizer Engineering Center Co., Ltd.

[0031] 2. Isolation and identification of strains

[0032] Add the soil samples collected in Hainan into sterile distilled water at a ratio of 1:10 (g / v), shake fully and let stand for 30 minutes, take 3ml supernatant and add rapeseed cake medium (10g rapeseed cake is added to 1L deionized water), and the enrichment solution was obtained after shaking culture at 30°C for 5 days. Take 0.5ml of the enrichment solution and spread it on the LB casein plate, and incubate at 30°C for 24h. Pick a single bacterial lawn, spot it on the LB casein medium plate, and observe whether there is a transparent circle after culturing for 24 hours. If a transparent circle is formed, measure the diameters of the transparent circle and the colony respectively, and calculate their ratio, and select the one with the larger ratio. stra...

Embodiment 2

[0033] Embodiment 2, the production of functional bacteria agent

[0034] Bacillus flexus NJNpd41 with strain preservation number CGMCC NO.7072 was inoculated into the fermentation broth for liquid fermentation production. The fermentation conditions were: pH 7.0, fermentation temperature range 30-35°C, stirring speed 180-200 rpm, The fermentation time is 24 hours, and the functional bacteria liquid is obtained, and the bacteria or spore content of the bacterial liquid is ≥10 10 pieces / ml. The preparation method of the fermentation medium used is as follows, taking the preparation of 1L medium as an example: glucose 10g, yeast extract 1g, (NH 4 ) 2 SO 4 1.0g, NaCl0.5g, the rest is water, pH7.2-7.5; sterilize at 121°C for 20min.

Embodiment 3

[0035] Embodiment 3, solid fermentation produces agricultural amino acid fertilizer

[0036] 3.1 Acquisition of solid fermentation process

[0037] Using the progressive method to obtain the solid fermentation process, first add the bacterial liquid to the rapeseed cake compost at different concentrations, the total weight of the fermentation heap is 2kg (dry weight), and ferment for 5 days to obtain the optimal bacterial liquid addition amount ( image 3 ), determine the daily amino acid changes to get the best composting days, and obtain the final solid fermentation process. The fermentation method is as follows: add 50L of functional bacteria liquid to each ton of solid organic material, turn the pile once a day during the solid fermentation process, and record the temperature of the pile ( Figure 4 ), when the temperature drops back to room temperature and the fermentation ends (different room temperature time varies), and finally the water content of the microbial organ...

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Abstract

The invention discloses a bactericide and process for producing an agricultural amino acid fertilizer bydetoxifying and hydrolyzing a rape seed cake. Bacillus flexus NJNpd41 is collected in China General Microbiological Culture Collection Center on 31, December 2012, with a collection number of CGMCC NO.7072. A method for producing the agricultural amino acid fertilizer by hydrolyzing the rape seed cake by using the NJNpd41 comprises the steps: regulating the content of the rape seed cake to 50 percent, and inoculating an NJNpd41 bacterium solution into the rape seed cake, and performing solid fermentation to obtain the agricultural amino acid fertilizer. By using the agricultural amino acid fertilizer produced by adopting the invention, the content of toxins contained in the rape seed cake can be reduced, the growth of an eggplant plant is remarkably promoted, the yield of eggplant is increased, and the quality of the eggplant is improved.

Description

technical field [0001] The invention relates to a bacterial agent and a process for producing agricultural amino acid fertilizer by detoxifying and hydrolyzing rapeseed cake. Background technique [0002] Rapeseed cake is rich in resources, and its crude protein content is about 37-42%, which is a valuable protein resource. 80% of the crude protein in rapeseed cake exists in the form of protein, and the remaining 20% ​​is free amino acid, erucic acid, nucleic acid, etc. Most of its protein is globulin, and the amino acid content is relatively balanced (Liu Zhenzhong's experimental research on amino acid hydrolyzate prepared from rapeseed cake). At present, many methods use acid hydrolysis to utilize amino acid resources in rapeseed cake, but the industrial cost is high and there is a large secondary pollution. The invention aims at screening protease-producing microorganisms and using the microorganisms to decompose vegetable cake protein into agricultural amino acid ferti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C05F11/08C12R1/07
CPCY02W30/40
Inventor 沈其荣李荣刘红军黄蓉文春燕张瑞福乔策策邵铖
Owner XINGHUA NEW SOIL SOURCE BASE FERTILIZER CO LTD
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