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Application of active ingredients of blackberry seeds

A technology of active ingredients, blackberry seeds, applied in the field of plant extract applications

Inactive Publication Date: 2016-04-13
HUANGHE S & T COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the effect of blackberry seeds on acute liver injury

Method used

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  • Application of active ingredients of blackberry seeds
  • Application of active ingredients of blackberry seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The extraction method of the effective components of blackberry seeds, the steps are as follows: crush 500g of blackberry seeds, use 4000mL of petroleum ether to degrease by cold soaking each time, repeat 4 times, each time 4d, filter, combine the extracts, recover petroleum ether from the extracts to obtain blackberry seed oil 145.04 g;

[0016] 341g of the blackberry seed residue after degreasing was soaked in 2800mL ethanol (70V%) for cold soaking, repeated 3 times, each time for 3 days, combined the ethanol extract, concentrated the ethanol extract to obtain the total ethanol extract, dispersed the total ethanol extract in water, and used Petroleum ether, ethyl acetate and n-butanol were extracted to obtain 2.34g of petroleum ether extract, 5.46g of ethyl acetate extract and 13.42g of n-butanol extract, which were respectively the petroleum ether part of blackberry seeds, ethyl acetate site and n-butanol site.

Embodiment 2

[0018] The method for extracting active ingredients of blackberry seeds, the steps are as follows: crush 500g of blackberry seeds, use 5000mL of petroleum ether to cold-soak and degrease each time, repeat 5 times, each time for 3 days, filter, combine the extracts, recover petroleum ether from the extracts to obtain blackberry seed oil 162.50 g;

[0019] 332g of the blackberry seed residue after degreasing was soaked in 3000mL ethanol (65V%) for cold soaking, repeated twice, each time for 4 days, combined the ethanol extract, concentrated the ethanol extract to obtain the total ethanol extract, dispersed the total ethanol extract in water, and used Petroleum ether, ethyl acetate and n-butanol were extracted to obtain 2.02g of petroleum ether extract, 5.10g of ethyl acetate extract and 14.28g of n-butanol extract, which were respectively the petroleum ether part of blackberry seeds, ethyl acetate site and n-butanol site.

Embodiment 3

[0021] The method for extracting the effective components of blackberry seeds is as follows: crush 500g of blackberry seeds, use 4500mL of petroleum ether to cold-soak and degrease each time, repeat 4 times, each time for 4 days, combine the extracts, recover petroleum ether from the extracts to obtain 162.26g of blackberry seed oil;

[0022] 335g of the blackberry seed residue after degreasing was soaked cold with 3300mL ethanol (80V%), repeated 3 times, 4 days each time, combined the ethanol extract, concentrated the ethanol extract to obtain the total ethanol extract, dispersed the total ethanol extract in water, and used Petroleum ether, ethyl acetate and n-butanol are extracted to obtain 2.26g of petroleum ether extract, 5.54g of ethyl acetate extract and 12.94g of n-butanol extract, which are respectively the petroleum ether part of blackberry seeds, ethyl acetate site and n-butanol site.

[0023] Effects of different parts of blackberry seeds on mice with acute liver in...

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Abstract

The invention belongs to the technical field of plant extract applications, in particular relates to a novel application of effective components of blackberry seeds and relates to applications in preparing a liver protecting health care product or drug and in the aspect of antioxidation. With the adoption of the novel application, a petroleum ether part, an ethyl acetate extract, an n-butyl alcohol extract and blackberry seed oil are extracted from the blackberry seeds; and studies confirm that the effective components of the blackberry seeds have certain protection action to acute liver injury of a carbon tetrachloride-induced mouse and is also used for strengthening the antioxidant function of a mouse organism with the acute liver injury and protecting the organism from being further damaged by free radicals.

Description

technical field [0001] The invention belongs to the technical field of application of plant extracts, and in particular relates to a new application of active ingredients of blackberry seeds. Background technique [0002] Blackberry (Rubusspp.Blackberry) belongs to the Rosaceae (Rasaceae) Rubus genus aggregate fruit plant, its fruit has high nutritional value and medicinal value, and has functions such as improving human metabolism, scavenging free radicals, delaying aging, and enhancing immunity , especially have the effects of lowering cholesterol content, preventing and treating heart disease, anti-cancer (reducing the toxic and side effects caused by chemotherapy) and anti-thrombosis. [0003] Blackberry seeds are by-products of processed products such as blackberry wine and blackberry juice. They are rich in polyphenolic compounds and have been largely abandoned for many years, resulting in a large waste of resources. In recent years, technicians have extracted blackbe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A23L33/105A61P1/16A61P39/06A23L33/00
Inventor 张伟康文艺尹震花
Owner HUANGHE S & T COLLEGE
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