Rice variety resource false smut resistance disease nursery identification method

An identification method and technology of rice false smut, which is applied in the field of plant disease resistance identification, can solve the problems of long artificial culture inoculum period, high risk, unstable artificial inoculation, difficult separation and purification of rice false smut, etc., to achieve identification and evaluation The results are true and reliable, good market application prospects and practical value, and the effect of good disease effect

Inactive Publication Date: 2014-07-16
HUNAN RICE RES INST
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Problems solved by technology

However, there are certain difficulties in the artificial inoculation of rice smut. The separation and purification of rice smut are difficult, and the growth rate is slow, which results in a long cycle of artificial culture inoculum and high risk, and the occurrence of artificial inoculation is unstable.
In addition, due to the large workload, this method is not suitable for the resistance identification of a large number of variety resources

Method used

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  • Rice variety resource false smut resistance disease nursery identification method

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Effect test

Embodiment 1

[0021] Site selection and establishment of embodiment 1 field natural disease nursery

[0022] A large number of studies have shown that chlamydospores and sclerotia of rice false smut can overwinter in fields and on weeds, and then can form a source of infection. Therefore, the rice smut nursery must first select the fields in the severely diseased areas that occur all year round, which can ensure the existence of a large number of primary infection sources. The occurrence of rice false smut also requires high temperature and high humidity environmental conditions, at a temperature of 25-32 °C and a humidity of more than 90%, the most suitable for the occurrence of rice false smut. In addition, the establishment of a disease nursery also needs to consider the flatness of the paddy field, good fertility, good irrigation conditions, easy operation and management, and convenient transportation.

[0023] In Hunan Province in 2004, due to the continuous rainy weather in the middl...

Embodiment 2

[0027] Example 2 Identification of resistance to rice smut disease in rice variety resources

[0028] This experiment was arranged in the disease nursery established in Example 1 in 2011. There were 22 rice materials for testing, namely WYQ12, 4123A / R6135, NA428, WYQ1, Xixuan 1798, WYQ7, Ningxiang 16, WYQ2, Xinnong Xiang21, Meixiangzhan, WYQ17, Yuzhenxiang, WYQ16, 386A / R6135, Wuyou 613, Nongxiang 26, Liangyou 937, Taifengyou 398, Huangyuezhan, Yuejingsimiao No. 2, Huangsizhan and Liangyoupeijiu ( susceptible control).

[0029] The rice materials for testing were sown on June 18, seedlings were raised in water, and transplanted to the diseased field on July 12. Each variety was planted with 30 plants, with a row spacing of 20cm×20cm, and repeated 3 times. Around the diseased garden (i.e. the four sides around the diseased garden field) plant 5 rows of rice smut disease-susceptible variety Liangyoupeijiu respectively; 20 days before the rice breach stage, the rice curved balls ...

Embodiment 3

[0035] Example 3 Identification of rice false smut resistance of regional test varieties in Hunan Province

[0036] This test was arranged in the disease nursery established in Example 1 in 2012. There were 112 10 groups of rice smut resistance identification varieties in the regional test in Hunan Province, including 58 varieties (5 groups) of medium indica and 11 varieties of one-season late indica. 1 variety (group 1), 23 varieties of double-season late indica (group 2), and 20 varieties of high-quality late indica (group 2).

[0037] Medium indica and one-season late indica were sown on June 9 and transplanted on July 2. Double-season late indica and high-quality late indica were sown on June 18 and transplanted on July 4. Insert 30 plants for each variety, the row spacing between plants is 20cm×20cm, and repeat 3 times. Around the disease nursery (that is, the four sides around the disease nursery field), 5 rows of rice smut susceptible variety Liangyoupeijiu were plante...

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Abstract

Disclosed is a rice variety resource false smut resistance disease nursery identification method. According to the method, a disease nursery is built on a piece of paddy field which is pathogenetic all year around in a region where false smut is popular; by planting pathogenetic varieties on the disease nursery, scattering false smut balls, increasing field humidity, unevenly applying nitrogen fertilizer and the like, rice is triggered to be pathogenetic, and a good pathogenetic effect can be acquired. Compared with prior artificial inoculation, identification of a large number of variety resources can be carried out at the same time, and the workload is greatly reduced. Complex work like separation, purifying and culture of artificially-inoculated pathogenic bacteria is avoided, and the identification technical difficulty is reduced. False smut resistance of a large number of rice variety resources, new strains, new combinations and breeding materials can be identified at the same time. The method provides evidence for false smut resistance resource selection, breeding for disease resistance, rice variety certification and the like, and the risk of an outbreak of false smut in rice planting is reduced. The method has good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of identification of plant disease resistance, specifically relates to the identification of resistance to rice false smut, in particular to a field-based natural disease nursery for the detection of rice false smut for large quantities of new rice varieties, combinations and germplasm resources. Methods for identification of resistance. Background technique [0002] Rice false smut is a fungal disease of rice spikes caused by Ustilaginoidea virens (Cooke) Tak, which has become one of the main diseases in various rice regions in China. Since the 1980s, the incidence area of ​​rice false smut in the whole country has increased significantly. For example, the incidence area in Jiangxi Province accounted for 30% of the total area of ​​rice in 1982; the incidence area in Guizhou Province accounted for 40% of the total area in 1982-1983; The diseased area accounts for 43% of the rice planting area and has a seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01G7/00A01G22/22
Inventor 唐善军高杜娟陈友德李友荣张世辉
Owner HUNAN RICE RES INST
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