Rice variety resource false smut resistance disease nursery identification method
An identification method and technology of rice false smut, which is applied in the field of plant disease resistance identification, can solve the problems of long artificial culture inoculum period, high risk, unstable artificial inoculation, difficult separation and purification of rice false smut, etc., to achieve identification and evaluation The results are true and reliable, good market application prospects and practical value, and the effect of good disease effect
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Embodiment 1
[0021] Site selection and establishment of embodiment 1 field natural disease nursery
[0022] A large number of studies have shown that chlamydospores and sclerotia of rice false smut can overwinter in fields and on weeds, and then can form a source of infection. Therefore, the rice smut nursery must first select the fields in the severely diseased areas that occur all year round, which can ensure the existence of a large number of primary infection sources. The occurrence of rice false smut also requires high temperature and high humidity environmental conditions, at a temperature of 25-32 °C and a humidity of more than 90%, the most suitable for the occurrence of rice false smut. In addition, the establishment of a disease nursery also needs to consider the flatness of the paddy field, good fertility, good irrigation conditions, easy operation and management, and convenient transportation.
[0023] In Hunan Province in 2004, due to the continuous rainy weather in the middl...
Embodiment 2
[0027] Example 2 Identification of resistance to rice smut disease in rice variety resources
[0028] This experiment was arranged in the disease nursery established in Example 1 in 2011. There were 22 rice materials for testing, namely WYQ12, 4123A / R6135, NA428, WYQ1, Xixuan 1798, WYQ7, Ningxiang 16, WYQ2, Xinnong Xiang21, Meixiangzhan, WYQ17, Yuzhenxiang, WYQ16, 386A / R6135, Wuyou 613, Nongxiang 26, Liangyou 937, Taifengyou 398, Huangyuezhan, Yuejingsimiao No. 2, Huangsizhan and Liangyoupeijiu ( susceptible control).
[0029] The rice materials for testing were sown on June 18, seedlings were raised in water, and transplanted to the diseased field on July 12. Each variety was planted with 30 plants, with a row spacing of 20cm×20cm, and repeated 3 times. Around the diseased garden (i.e. the four sides around the diseased garden field) plant 5 rows of rice smut disease-susceptible variety Liangyoupeijiu respectively; 20 days before the rice breach stage, the rice curved balls ...
Embodiment 3
[0035] Example 3 Identification of rice false smut resistance of regional test varieties in Hunan Province
[0036] This test was arranged in the disease nursery established in Example 1 in 2012. There were 112 10 groups of rice smut resistance identification varieties in the regional test in Hunan Province, including 58 varieties (5 groups) of medium indica and 11 varieties of one-season late indica. 1 variety (group 1), 23 varieties of double-season late indica (group 2), and 20 varieties of high-quality late indica (group 2).
[0037] Medium indica and one-season late indica were sown on June 9 and transplanted on July 2. Double-season late indica and high-quality late indica were sown on June 18 and transplanted on July 4. Insert 30 plants for each variety, the row spacing between plants is 20cm×20cm, and repeat 3 times. Around the disease nursery (that is, the four sides around the disease nursery field), 5 rows of rice smut susceptible variety Liangyoupeijiu were plante...
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