In-vitro rapid propagation method for ornithogalum caudatum by taking infant flowers as explants

An explant, evergreen technology, applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of low reproduction coefficient, limited application of tiger eye evergreen tissue culture technology, unfavorable subculture, etc., and achieve regeneration. High efficiency, low cost and neat appearance

Inactive Publication Date: 2014-07-23
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the previous experiments on the evergreen tissue culture have been initially successful, bulbs are usually used as explants to induce callus. Because bulbs are used as explant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Explant inoculation and callus induction: When the size of the flower buds has been or nearly fully developed but is still green, cut off the flower buds on the young inflorescence from the lower end of the ovary, and put the complete flower buds into a sterile ultra-clean In Taichung, soak in 75% (volume / volume) alcohol for 60 seconds, soak in 0.1 (mass / volume) mercury chloride solution for 10 minutes, and rinse with sterile water 5 times. The bud is cut from the upper part of the ovary, then most of the upper part of the bud is cut off, and the remaining part is cut longitudinally twice, and inoculated in a medium containing 6-BA2.0mg / L, NAA0.1mg / L, sucrose 4% (mass / volume) , on MS medium with a pH value of 6.2, irradiated with fluorescent light for 4 to 6 hours a day at 20°C to 30°C, and the light intensity is 1500 to 2000 Lux. Subculture once every 25-35 days, and induce differentiation when the yellow-green callus grows.

[0013] 2. Induction of adventitious bu...

Embodiment 2

[0017] 1. Explant inoculation and callus induction: When the size of the flower buds has been or nearly fully developed but is still green, cut off the flower buds on the young inflorescence from the lower end of the ovary, and put the complete flower buds into a sterile ultra-clean In Taichung, soak in 75% (volume / volume) alcohol for 90 seconds, soak in 0.2% (mass / volume) mercury chloride solution for 8 minutes, and rinse with sterile water 5 times. The flower bud is cut off from the upper part of the ovary, then most of the upper part of the flower bud is cut off, the remaining part is cut longitudinally, and inoculated in a medium containing 6-BA1.5mg / L, NAA0.15mg / L, sucrose 3.5% (mass / volume), On MS medium with a pH value of 5.9, irradiate with fluorescent lamp for 4 to 6 hours every day at 20°C to 30°C, and the light intensity is 1500 to 2000 Lux. Subculture once every 25-35 days, and induce differentiation when the yellow-green callus grows.

[0018] 2. Induction of adv...

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PUM

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Abstract

The invention belongs to a flower and plant propagation method and particularly relates an asexual rapid propagation method for flowers and plants. In the asexual rapid propagation method, infant flower base parts of ornithogalum caudatum infant inflorescences are taken as explants for carrying out tissue culture; the de-differentiation rate and the differentiation rate can be up to more than 75%. A callus which is induced by one explant can be subjected to repeated subculture so as to differentiate about 500 cluster buds; the phenotypes of the cluster buds are uniform. The rooting rate of the cluster buds can be up to more than 90% and the transplanting survival rate under a suitable environment is up to 100%. The ornithogalum caudatum can be propagated into plants with completely same genetic backgrounds and ordered appearances in a large scale within a relative short time; the variation of regenerated plants is low; a regeneration system is very efficient; the cost of an average single-plant regenerated seedling is very low; the asexual rapid propagation method is suitable for being used in large-batch, efficient and rapid propagation of the ornithogalum caudatum and is very suitable for being used in industrial production.

Description

technical field [0001] The invention belongs to flower propagation method, in particular to the asexual rapid propagation method of flower. Background technique [0002] Evergreen tiger eye, also known as squill, bird's milk flower. It is a perennial herb of the Liliaceae evergreen genus, native to southern Africa. Bulbs ovoid, green, up to 10 cm in diameter. Leaves 5-6 pieces, band-shaped or strip-shaped-lanceolate, 30-60 cm long, 2.5-5 cm wide. As early as the 1970s, tiger-eye evergreen was introduced to China as an ornamental plant, and it was planted in Hainan, Xi'an, and Changbai Mountains. After the experimental research of many scholars, it was found that Dieffenbachia saponin (OSW-1 for short) was isolated from Dieffenbachia tiger's eye. In the physiological activity screening experiment, it was found that the in vitro anticancer activity of "OSW-1" was ten times or even a hundred times stronger than the clinically used anticancer drugs such as paclitaxel, doxoru...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 崔金腾张克中李月华王杰田娜
Owner BEIJING UNIV OF AGRI
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