A kind of content determination method of cockles
A determination method and clam technology, applied in measuring devices, color/spectral characteristic measurement, instruments, etc., can solve problems of inability to guarantee product quality, inaccurate determination of protein and polysaccharide content, lack of specificity and content determination of clam polypeptide and polysaccharide Reporting and other issues to achieve specific, effective and quality-controllable results
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Embodiment 1
[0044] Take fresh cockle meat, crush it, pass through a 40-mesh sieve, add 1 times the amount of water, stir and extract at below 10°C for 4 hours, filter the extract, and centrifuge. The above-mentioned centrifuged solution was freeze-dried to obtain the clam extract.
[0045] There is a sharp characteristic peak "Peak S" at the retention time of 15.564min in the characteristic spectrum of the clam extract. Its molecular weight is 88070. Satisfy the specificity of clam polypeptide, proceed to the following operations;
[0046] There are 2 characteristic peaks in the characteristic spectrum of the clam extract. Taking the S peak in the spectrum as 1, the relative retention time of peak 1 is 0.57, and the relative retention time of peak 2 is 0.68. Its molecular weight is 696214, which meets the specificity of the clam polysaccharide.
[0047] At the same time, it meets the specificity of clam polypeptide and polysaccharide, indicating that it is made from clam, and the conten...
Embodiment 2
[0052] Take dry clam meat, crush it, pass it through a 80-mesh sieve, add 10 times the amount of water, heat and extract twice, 1 hour each time, combine the extracts, filter, take the filtrate, and concentrate to a relative density of 1.04-1.06 (60°C) , centrifuge (2000 rpm). Take the above-mentioned centrifuged solution below 80°C, dry it under reduced pressure, and pulverize it to obtain the clam extract. The extract is prepared into tablets.
[0053] Take the tablet to identify the clam polypeptide, and there is a sharp characteristic peak "Peak S" at the retention time of 15.239min in the characteristic map. Its molecular weight is 88070. Satisfy the specificity of clam polypeptide, proceed to the following operations;
[0054] Take the tablet to identify the clam polysaccharides. There are 2 characteristic peaks in the characteristic spectrum. Taking the S peak in the spectrum as 1, the relative retention time of peak 1 is 0.59, and the relative retention time of peak...
Embodiment 3
[0060] The clam extract was prepared according to the preparation method of the clam extract in Example 2, and the extract was made into capsules.
[0061] The contents of the capsule were taken to identify the clam polypeptide. The results showed that there was a sharp characteristic peak "Peak S" at the retention time of 15.933min in the characteristic spectrum of the clam extract, and its molecular weight was 88070. Satisfy the specificity of clam polypeptide, proceed to the following operations;
[0062] The contents of the capsules were taken to identify the clam polypeptides. There were two characteristic peaks in the characteristic spectrum. Taking the S peak in the spectrum as 1, the relative retention time of peak 1 was 0.53, and the relative retention time of peak 2 was 0.63. Polysaccharide specificity.
[0063] At the same time, it meets the specificity of clam polypeptide and polysaccharide, indicating that it is made from clam, and the content determination is co...
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