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Use of annelid haemoglobin for maintaining stem cells in the undifferentiated state

A hemoglobin, annelid technology, applied in animal cells, vertebrate cells, non-embryonic pluripotent stem cells, etc.

Active Publication Date: 2014-08-27
埃玛里纳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, there are medical applications (compensating for the deficiencies of certain organs), but there are also pharmacological applications; and finally

Method used

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  • Use of annelid haemoglobin for maintaining stem cells in the undifferentiated state
  • Use of annelid haemoglobin for maintaining stem cells in the undifferentiated state

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] Materials and Methods

[0035] active agent

[0036] Extracellular hemoglobin from annelids (in this case the green clamworm) was used at test concentrations of 0.25, 0.75 and 1.25 mg / mL in a buffer solution containing 90 mM NaCl, 23 mM sodium gluconate, 2.5 mM CaCl 2 , 27mM sodium acetate, 1.5mM MgCl 2 , 5 mM KCl, and a pH of 7.1±0.5, which may contain 0-100 mM ascorbic acid and / or reduced glutathione type antioxidants. This hemoglobin is commercially available from Hemarina Corporation under the reference number Hemarina-M201.

[0037] CFE Clonogenic Assay (2D)

[0038]Rationale: The CFE Clonogenic Efficacy Assay assesses the frequency of keratinocytes capable of producing cell clones within a given population. A strong clonogenic potential is thought to be associated with stem / progenitor cells, whereas differentiated keratinocytes lose this potential. This culture test consists in seeding keratinocytes at a low density (100 to 1000 cells / dish) in order to o...

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Abstract

The invention relates to the use of at least one extracellular globin, globin protomer or haemoglobin from annelids, for maintaining stem cells in the undifferentiated state.

Description

technical field [0001] The present invention relates to the use of at least one extracellular hemoglobin, globulin or proglobulin from annelids for maintaining stem cells in an undifferentiated state. Background technique [0002] Today, stem cells represent a valuable therapeutic potential, especially from the point of view of regenerative or therapeutic medicine. Together they make up a variety of specific cell classes: [0003] - Adult stem cells: originally discovered as blood cells. They are widely used after chemotherapy that destroys bone marrow cells. An autologous transplant (of the patient's own) enables faster production of blood cells without the risk of rejection. All tissues and all organs contain adult stem cells, but they are rare and difficult to purify; [0004] - Perinatal stem cells: they are contained in the umbilical cord, the placenta and the blood containing it. Mention may be made of blood stem cells, which may be used for transplantation, but s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/075A01N1/02
CPCC12N2501/998C12N5/0629C12N5/063C12N5/0607A01N1/0226
Inventor F·扎尔
Owner 埃玛里纳
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