Rhodamine-containing lactam group micromolecule pH fluorescent probe and synthetic method
A fluorescent probe, Minglactam technology, applied in chemical instruments and methods, luminescent materials, organic chemistry, etc., can solve problems such as hydrogen ion fluorescence response interference, and achieve the effect of good anti-interference ability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] (1) Synthesis of compound 1:
[0024] Dissolve 2.395g of rhodamine B (5mmol) in 50mL of absolute ethanol, and then add 1.5g of ethylenediamine. The reaction system was stirred at reflux for 6 hours. After the reaction was completed, the solvent was evaporated under reduced pressure, and the remaining solid was dissolved in 200mL of 1mol / L HCl solution, and 1mol / L NaOH solution was added dropwise to pH=9-10. A large amount of solid precipitated, filtered under reduced pressure, washed with water, and then pumped thoroughly. After drying in a vacuum oven, a brick-red solid was obtained, namely compound 1
[0025] (2) Synthesis of pH fluorescent probe:
[0026] In a 50 mL round bottom flask, 0.485 g of compound 1 (1 mmol) dissolved in 15 mL of methanol was added, and then 0.127 g of p-hydroxybenzaldehyde (1 mmol) was added, and the mixture was refluxed for 4 h. After the reaction, the solid was cooled and precipitated, filtered, washed with methanol until the filtrate b...
Embodiment 2
[0029] Draw the fluorescent emission spectrum of the small molecule pH probe containing the rhodamine lactam group obtained in Example 1 at different pHs in the aqueous solution, the concentration is 0.16 mg / mL, the abscissa is pH, and the ordinate is fluorescence intensity. figure 2 .
[0030] It can be seen from the figure that when the probe is at pH=4-6, its fluorescence intensity has a sudden change, and it can be seen that the fluorescence response range is at pH=4-6. And the response interval is relatively narrow, indicating that the probe pair H + The fluorescence response is very sensitive.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com