A polymer fluorescent probe containing rhodamine lactam group in the detection of h + application on

A fluorescent probe and polymer technology, applied in the field of polymer fluorescent probes, can solve problems such as hydrogen ion fluorescence response interference, and achieve the effects of good anti-interference ability and good water solubility

Inactive Publication Date: 2017-05-03
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, most rhodamine lactam compounds will react with some metal ions such as coordination or catalytic decomposition, so the fluorescence response to hydrogen ions is interfered

Method used

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  • A polymer fluorescent probe containing rhodamine lactam group in the detection of h <sup>+</sup> application on
  • A polymer fluorescent probe containing rhodamine lactam group in the detection of h <sup>+</sup> application on
  • A polymer fluorescent probe containing rhodamine lactam group in the detection of h <sup>+</sup> application on

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Synthesis of compound 1:

[0026] Dissolve 2.395g of rhodamine B (5mmol) in 50mL of absolute ethanol, and then add 1.5g of ethylenediamine. The reaction was stirred at reflux for 6 hours. After the reaction was completed, the solvent was evaporated under reduced pressure, and the remaining solid was dissolved in 200mL of 1mol / L HCl solution, and 1mol / L NaOH solution was added dropwise to pH=9-10. A large amount of solid precipitated, filtered under reduced pressure, washed with water, and then thoroughly pumped. After drying in a vacuum oven, a brick-red solid was obtained, namely compound 1.

[0027] (2) Synthesis of Compound 2:

[0028] Add 0.968g of compound 1 (2mmol) into a 50mL three-necked flask, dissolve it in 20mL of dichloromethane, then add 0.3mL of triethylamine, slowly add 0.2g of acryloyl chloride and 0.2g (2.2mmol) of dichloromethane in an ice bath 5mL, continue to react at room temperature for 6 hours after dropping. After the reaction was complet...

Embodiment 2

[0034] (1) Draw the ultraviolet-visible absorption spectrum of the polymeric pH probe containing the rhodamine lactam group obtained in Example 1 at different pHs, the concentration is 0.16mg / mL, the abscissa is pH, and the ordinate is absorbance ,have to figure 1 ;

[0035] It can be seen from the figure that when the probe is at pH=4.2-6.5, its absorbance value will change suddenly, and it can be seen that the color response range of the probe solution is at pH=4.2-6.5.

[0036] (2) Draw the fluorescence emission spectrum of the polymer pH probe containing the rhodamine lactam group gained in Example 1 at different pHs in the aqueous solution, the concentration is 0.16mg / mL, the abscissa is pH, and the ordinate is fluorescence intensity, have to figure 2 .

[0037] It can be seen from the figure that when the probe is at pH=4-5, its fluorescence intensity has a sudden change, and it can be seen that the fluorescence response range is at pH=4-5. And the response interval...

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Abstract

The invention relates to the application of a polymer fluorescent probe containing rhodamine lactam group in detecting H+. The synthesis method is to react rhodamine with a carboxyl group at the 2-position with ethylenediamine to form lactam, and then mix with The material reaction of acryloyl chloride group produces a monomer containing a rhodamine group, and finally it is copolymerized with a substance containing an acrylamide group to synthesize a polymer pH fluorescent probe that has a specific fluorescent response to H+ and can enter living cells .

Description

technical field [0001] The invention relates to a polymer fluorescent probe containing a rhodamine lactam group in the detection of H + on the application. Background technique [0002] Rhodamine fluorescent dye molecules with a lactam spiro ring structure can have a very strong response ability. After interacting with specific ions, they can change from a non-fluorescent and colorless lactam spiro ring state to a fluorescent quantum efficiency of more than 85% and Open loop states with dark features. This means that this rhodamine-like molecule has a good performance basis. Moreover, rhodamine-based materials themselves have been widely used in the fields of biology and the environment, and have a good application basis. However, in the biological field, intracellular pH detection reagent materials often have stricter requirements. Although there are many reports in the literature on new materials for pH probes, most of the probes currently lack sufficient practicabilit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C08F220/56C08F220/28C08F220/60G01N21/64
Inventor 王红霞戚裕朱威周梦超邵晶
Owner SUZHOU UNIV OF SCI & TECH
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