Method for expansion of dynamic range of biochip detector and concentration back calculation based on fractional exposure
A biochip and dynamic range technology, which is applied in the direction of material analysis, instruments, and measuring devices through optical means, and can solve problems such as splicing and unusable images
Active Publication Date: 2014-10-01
上海昌润生物科技有限公司
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To solve this problem, you can use the method of sub-exposure similar to surveillance cameras, but the difference is that you cannot use the method of image stitching
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[0021] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0022] The biochip is marked with 0, 1, 2, 3, 4, a total of 5 standard products with increasing concentrations, as well as quality control products and patient serum.
[0023] Use two exposure times of N1 seconds and N2 seconds (N1<N2) to shoot sequentially. The principle for determining the exposure time N1 second and N2 second is: on the image exposed at N1 second, the high-density part will not be saturated, and on the image exposed at N2 second, the low-density part can be seen clearly, and the signals marked 1 and 2 The value should be able to be pulled apart, and the signal value marked with 4 is about 2 / 3 of the full scale.
[0024] The whole significance of the image is that the concentration of each indicator in the patient's serum depends on the comparison between the gray value on the image and the gray value of the standard product...
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The invention discloses a method for expansion of the dynamic range of a biochip detector and concentration back calculation based on fractional exposure. The method includes the following steps that firstly, fractional exposure time values are determined according to gray scale intensity, shooting is conducted sequentially at exposure time values from small to large, images of standard substances at different exposure time values are obtained, and then the gray scale values of the standard substances at different exposure time values are obtained; secondly, the gray scale values of the standard substances at different exposure time values serve as Y-axis values and the concentrations of the standard substances serve as x-axis values, curve fitting is conducted, and fitted curves of the standard substances at different exposure time values are obtained; thirdly, according to the method that the fitted curves of the standard substances at every two adjacent exposure time values pass the same fixed point, the fitted curves of the standard substances at different exposure time values are spliced seamlessly to form a continuous curve in monotone increasing; meanwhile, the same fixed point is set as a demarcation point; fourthly, the gray scale value of a sampled blood serum is compared with the gray scale value at the demarcation point, and back calculation is conducted on the concentration of the sampled blood serum according to the fitted curves of the standard substances at the determined exposure time value.
Description
technical field [0001] The invention relates to a method for expanding the dynamic range of a biological chip detector and recalculating the concentration through multiple exposures. The method is to organically combine two working curves obtained by the multiple exposures, and then recalculate the concentration. This method expands the concentration range that the biochip detector can detect. Background technique [0002] The key device of the biochip detector is the CCD, and the dynamic range of the CCD determines the detection range of the detector. [0003] The CCD dynamic range means that the CCD can clearly distinguish the illuminance ratio of the brightest and darkest parts of the image at the same time. The larger the dynamic range, the richer and clearer the layers displayed by the image, and the wider the color space of the image. [0004] The dynamic range of the human eye is very large, the human eye can focus on one point, and then see a very dark place clearl...
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IPC IPC(8): G01N21/00
Inventor 张香琴姚涌
Owner 上海昌润生物科技有限公司