Feed additive and preparation method thereof

A technology of feed additives and raw materials, applied in the field of high-efficiency feed additives and its preparation, can solve problems such as difficulty in realizing industrialization, standardization, impact on feed nutrition ratio, and simple production equipment, so as to enhance cellular immunity and humoral immunity, improve daily Weight gain and feed conversion ratio, the effect of less usage

Active Publication Date: 2014-10-08
BEIJING DORUN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the Chinese herbal medicine feed additives put on the market are powders or powders. Their production technology is backward, the production equipment is simple, the processing is rough and simple, the variety is single, and the dosage is generally too large. This not only increases the product cost, wastes me

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] (1) Preparation of bacterial suspension

[0038] The single colony of Li-2013 grown on the plate streaked and separated into the seed culture medium, 100r / min, 40 ℃ after 12 hours of culture, 1mL of culture broth was centrifuged and washed twice with physiological saline, and resuspended with 9mL physiological In salt water.

[0039] (2) UV-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis

[0040] Place the bacterial suspension in a sterile plate, stir and irradiate it under a UV lamp with a distance of 30cm and a power of 15w for 100s. The irradiated bacterial solution was applied to a lithium chloride plate after gradient dilution, and the plate was diluted with the bacterial solution without ultraviolet irradiation as a control. Wrap the above-mentioned uniformly coated plate with black cloth or newspaper, and incubate at 40°C for 48 hours. On the plate with colony growth, the one with the largest ratio of hydrolysis circle to colony diameter is selected and stored o...

Example Embodiment

[0057] Example 1: The preparation method is the same as Example 3

[0058] A feed additive includes the following raw materials in parts by weight: 27 parts of Astragalus extract; 15 parts of Codonopsis pilosula extract; 16 parts of Bupleurum extract; and 20 parts of Bacillus subtilis culture.

Example Embodiment

[0059] Example 2:

[0060] Preparation of Bacillus subtilis culture:

[0061] Stepwise expansion of slant strains to obtain fermentation broth of Bacillus subtilis;

[0062] (1) First-level seed culture: Put the Bacillus subtilis slant strain into a 500 ml shake flask, fill the medium with 100 ml, rotate the shaker at 180 rpm, culture temperature 40°C, culture time 12 hours;

[0063] (2)Secondary seed culture: Put the first-level seed into a 500ml second-level seed shaker at 10% of the inoculum, and the cultivation conditions are the same as those of the first-level seed;

[0064] (3) Three-level seed culture: Put the second-level seed into a 5000 ml third-level seed shaker at 10% inoculum, the medium is 1000 ml, the rotating shaker is 100 rpm, the culture temperature is 40 ℃, and the culture Time 12 hours;

[0065] (4) First-level seed tank culture: Connect the third-level seeds to a first-level seed tank with a total volume of 150L at 10% of the inoculum. The fermentation medium is lo...

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Abstract

Belonging to the field of feed additives, the invention discloses a feed additive and a preparation method thereof. The feed additive comprises the following raw materials by weight: 20-35 parts of an astragalus extract; 10-20 parts of a Codonopsis pilosula extract; 10-20 parts of a radix bupleuri extract; and 10-25 parts of a bacillus subtilis culture. The product provided by the invention has the characteristics of small usage, stable performance, use safety, and no incompatibility with other feed additives. The Chinese herbal extracts and a microbial agent are adopted in a reasonable proportion, enzymolysis and ethanol immersion extraction are employed to improve the extraction rates of amino acid, polysaccharide, trace elements and other beneficial ingredients in Chinese herbal medicines, and then by combining with the bacillus subtilis culture with high enzyme yield, digestion and absorption of beneficial Chinese herbal ingredients in feed can be promoted effectively. At the same time, the usable value of energy and protein of various feed resources is also enhanced, the production ability, the stress response and raising comprehensive benefits of livestock and poultry are strengthened, the animal daily gain and feed conversion rate are improved, and the drug usage is saved.

Description

Technical field: [0001] The invention belongs to the field of feed additives, in particular to a high-efficiency feed additive containing various traditional Chinese medicine components and a preparation method thereof. Background technique: [0002] With the rapid development of animal husbandry in our country, the application of feed additives has become more and more extensive, which has made great contributions to ensuring the healthy development of animal husbandry and meeting people's demand for animal food, but it has also brought a series of problems. Since most of the additives belong to chemically synthesized drugs, such as hormones, antibiotics, etc., while increasing the production of livestock and poultry, the drug residues in livestock products increase, which directly endangers human health; and in the process of modern livestock and poultry breeding, in order to prevent And to treat animal diseases, excessive antibiotic products are often used to ensure anima...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14A23K1/00A23K10/12A23K20/10A23K20/189
Inventor 朱建国王剑顾学珠夏圣荣伍华东
Owner BEIJING DORUN TECH
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