Feed additive and preparation method thereof
A technology of feed additives and raw materials, applied in the field of high-efficiency feed additives and its preparation, can solve problems such as difficulty in realizing industrialization, standardization, impact on feed nutrition ratio, and simple production equipment, so as to enhance cellular immunity and humoral immunity, improve daily Weight gain and feed conversion ratio, the effect of less usage
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[0037] (1) Preparation of bacterial suspension
[0038] The single colony of Li-2013 grown on the plate streaked and separated into the seed culture medium, 100r / min, 40 ℃ after 12 hours of culture, 1mL of culture broth was centrifuged and washed twice with physiological saline, and resuspended with 9mL physiological In salt water.
[0039] (2) UV-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis
[0040] Place the bacterial suspension in a sterile plate, stir and irradiate it under a UV lamp with a distance of 30cm and a power of 15w for 100s. The irradiated bacterial solution was applied to a lithium chloride plate after gradient dilution, and the plate was diluted with the bacterial solution without ultraviolet irradiation as a control. Wrap the above-mentioned uniformly coated plate with black cloth or newspaper, and incubate at 40°C for 48 hours. On the plate with colony growth, the one with the largest ratio of hydrolysis circle to colony diameter is selected and stored o...
Example Embodiment
[0057] Example 1: The preparation method is the same as Example 3
[0058] A feed additive includes the following raw materials in parts by weight: 27 parts of Astragalus extract; 15 parts of Codonopsis pilosula extract; 16 parts of Bupleurum extract; and 20 parts of Bacillus subtilis culture.
Example Embodiment
[0059] Example 2:
[0060] Preparation of Bacillus subtilis culture:
[0061] Stepwise expansion of slant strains to obtain fermentation broth of Bacillus subtilis;
[0062] (1) First-level seed culture: Put the Bacillus subtilis slant strain into a 500 ml shake flask, fill the medium with 100 ml, rotate the shaker at 180 rpm, culture temperature 40°C, culture time 12 hours;
[0063] (2)Secondary seed culture: Put the first-level seed into a 500ml second-level seed shaker at 10% of the inoculum, and the cultivation conditions are the same as those of the first-level seed;
[0064] (3) Three-level seed culture: Put the second-level seed into a 5000 ml third-level seed shaker at 10% inoculum, the medium is 1000 ml, the rotating shaker is 100 rpm, the culture temperature is 40 ℃, and the culture Time 12 hours;
[0065] (4) First-level seed tank culture: Connect the third-level seeds to a first-level seed tank with a total volume of 150L at 10% of the inoculum. The fermentation medium is lo...
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