Method for detecting blood sugar-lowering and blood pressure-lowering drugs in blood by ultra high performance liquid-mass spectrometry

A technology of ultra-high performance liquid chromatography and mass spectrometry, which is applied in the field of detection of hypoglycemic and hypotensive drugs in blood by ultra-high performance liquid chromatography-mass spectrometry, which can solve the problems of limited detection time, complicated extraction process, and small amount of biological samples

Active Publication Date: 2014-11-05
中国民用航空局民用航空医学中心
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  • Abstract
  • Description
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Problems solved by technology

Although document 6 also uses ultra-high performance liquid chromatography-mass spectrometry, there are only two types of drugs to be detected, and there are few types, and the sample pretreatment uses solid-phase extraction, which is complicated and time-consuming.
[0006] There are different types of antihypertensive and hypoglycemic drugs commonly used at home and abroad, and the above-mentioned detection methods in the prior art cannot cover the types of drugs commonly used in China
In addition, due to the large number of test subjects (in addition to more than 30,00

Method used

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  • Method for detecting blood sugar-lowering and blood pressure-lowering drugs in blood by ultra high performance liquid-mass spectrometry
  • Method for detecting blood sugar-lowering and blood pressure-lowering drugs in blood by ultra high performance liquid-mass spectrometry
  • Method for detecting blood sugar-lowering and blood pressure-lowering drugs in blood by ultra high performance liquid-mass spectrometry

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Experimental program
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Effect test

Embodiment 1

[0134] Embodiment 1 establishes the detection method of hypoglycemic and antihypertensive drugs in blood

[0135] 1.1 Establishment of detection target compounds and basic properties of compounds

[0136] See Table 6 for the chemical properties of 31 commonly used antihypertensive and hypoglycemic drugs; see Tables 7-1 and 7-2 for drug indications, adverse reactions, metabolic half-lives reported in literature, minimum detection limits, and minimum quantification limits.

[0137] Table 6 List of chemical properties of target compounds

[0138]

[0139]

[0140]

[0141]

[0142]

[0143] Table 7-1, half-life, minimum detection limit, and minimum quantification limit of 31 drugs

[0144]

[0145]

[0146]

[0147] Table 6-2, List of indications and adverse reactions of 31 drugs

[0148]

[0149]

[0150] 1.2 Establishment of target compound detection instrument method

[0151] 1.2.1 Establishment of UPLC-MS / MS detection method

[0152] 1.2.1.1 ...

Embodiment 2

[0184] Example 2 Application of blood sugar-lowering and antihypertensive drug detection method

[0185] In order to test the accuracy of the ultra-high performance liquid chromatography-mass spectrometry method established in Example 1 to detect the results of hypoglycemic and antihypertensive drugs in blood samples, we collected blood samples from 14 cases of clinical patients in Civil Aviation Hospital. Antihypertensive and hypoglycemic drugs UPLC-MS / MS detection method was used to screen and detect hypoglycemic and antihypertensive drugs. The results are shown in Table 8.

[0186] Table 8. Detection table of hypoglycemic and hypotensive drugs in the blood of clinical patients

[0187]

[0188]

[0189] From the above results, it can be seen that the test results are basically consistent with the information on the medication prescribed by the doctor, indicating that the established liquid chromatography-mass spectrometry method for the detection of hypoglycemic and ...

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Abstract

The invention provides a method for detecting 31 common blood pressure-lowering and blood sugar-lowering drugs in blood by an ultra high performance liquid-mass spectrometry, and is characterized in that the method comprises the following steps: (1) confirmation of detected target compounds; (2) sample processing: processing a sample by an organic solvent protein-deposition method; (3) primary screening detection: carrying out primary screening detection by a multiple reaction monitoring (MRM) mode of the ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in different periods of time; (4) reexamining of the sample which is positive by the primary screening detection in the step (3): after treating and concentrating the sample which is positive by the screening detection, carrying out UPLC-MS/MS detection; and optionally, (5) reexamining of the sample which is positive by the reexamining detection in the step (4): reexamining by a high performance liquid chromatography-high resolution time-of-flight tandem mass spectrometry (HPLC-QTOF). According to a fact that the drugs have different retention time in the set detection conditions, a method for sectional detection is creatively completed. The required time for totally completing the detection of 31 drugs can be within 11 minutes.

Description

technical field [0001] The invention relates to a method for detecting hypoglycemic and hypotensive drugs in blood by ultra-high performance liquid phase-mass spectrometry. Background technique [0002] Flight safety is a major issue related to the national economy and the people's livelihood. Hypertension and diabetes are common diseases among pilots. They are the basic diseases of many disabling diseases, and they are also important reasons for pilots to be grounded. Rational use of drugs is still an important measure for the treatment of hypertension and diabetes, so as to prevent heart, kidney, brain, nervous system and other complications caused by hypertension and diabetes, and prolong the flight life of pilots; however, antihypertensive drugs and hypoglycemic drugs Unreasonable use may lead to a sharp drop in blood pressure and blood sugar, resulting in sudden disability of the pilot. According to the "Civil Aviation Personnel Physical Examination Certificate Managem...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 李清艳乔湜李松林许海山王伟徐先发刘国如于红燕丁仁奎刘永锁
Owner 中国民用航空局民用航空医学中心
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