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A high-performance liquid phase-high resolution time-of-flight tandem mass spectrometry method for detecting hypoglycemic and hypotensive drugs in blood

A time-of-flight and tandem mass spectrometry technology, which is applied in the direction of measuring devices, material separation, and analysis of materials, to achieve the effect of improving accuracy

Active Publication Date: 2016-05-11
中国民用航空局民用航空医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, there is still a lack of quick, easy, sensitive, reliable, reproducible and accurate methods for detecting common blood pressure and blood sugar lowering drugs that can be used for pilots' medication conditions.

Method used

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  • A high-performance liquid phase-high resolution time-of-flight tandem mass spectrometry method for detecting hypoglycemic and hypotensive drugs in blood
  • A high-performance liquid phase-high resolution time-of-flight tandem mass spectrometry method for detecting hypoglycemic and hypotensive drugs in blood
  • A high-performance liquid phase-high resolution time-of-flight tandem mass spectrometry method for detecting hypoglycemic and hypotensive drugs in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Embodiment 1 establishes the detection method of hypoglycemic and antihypertensive drugs in blood

[0118] 1.1 Establishment of detection target compounds and basic properties of compounds

[0119] See Table 5 for the chemical properties of 31 commonly used antihypertensive and hypoglycemic drugs; see Tables 6-1 and 6-2 for drug indications, adverse reactions, metabolic half-lives reported in literature, minimum detection limits, and minimum quantification limits.

[0120] Table 5. List of chemical properties of target compounds

[0121]

[0122]

[0123]

[0124]

[0125]

[0126] Table 6-1, list of half-life, minimum detection limit and minimum quantification limit of 31 drugs

[0127]

[0128]

[0129]

[0130] Table 6-2, List of indications and adverse reactions of 31 drugs

[0131]

[0132]

[0133]

[0134] 1.2 Establishment of target compound HPLC-QTOF detection instrument method

[0135] 1.2.1 Reagents and instruments

[0136...

Embodiment 2

[0185] Embodiment 2 The application of blood hypoglycemic and antihypertensive drug detection method of the present invention

[0186] In order to test the accuracy of the results of the liquid chromatography mass spectrometry established in Example 1 in detecting blood sugar and hypotensive drugs in blood samples, we collected blood samples from 14 cases of clinical patients in civil aviation hospitals, and used the common blood pressure-lowering, The HPLC-QTOF detection method of hypoglycemic drugs was used to screen and detect hypoglycemic and antihypertensive drugs. The results are shown in Table 8.

[0187] Table 8. Detection table of hypoglycemic and hypotensive drugs in the blood of clinical patients

[0188]

[0189]

[0190] From the above results, it can be seen that the test results are basically consistent with the information on the medication prescribed by the doctor, indicating that the established liquid chromatography-mass spectrometry method for the de...

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Abstract

The invention provides a method for detecting a drug capable of reducing blood sugar and blood pressure by high-performance liquid chromatography-high resolution time of flight tandem mass spectrometry. The method comprises the following steps of (1) identifying a to-be-detected target compound; (2) pre-treating a sample: treating the sample by using an organic solvent precipitation protein method; (3) detecting by using the high-performance liquid chromatography-high resolution time of flight tandem mass spectrometry (HPLC-QTOF) and screening and analyzing according to a retention time, accurate molecular weight and isotopic peak abundance; (4) re-checking the positive samples detected by the step (3); and / or (5) re-detecting by using HPLC-QTOF and identifying according to the retention time and the accurate molecular weight. According to different retention times of each drug in detection conditions set by the method, the method that carries out detection in section is finished originally. The method can finish the detection of 31 drugs within 20 minutes. Detection accuracy of the method can reach 95%, preferably, over 99%.

Description

technical field [0001] The invention relates to a method for detecting hypoglycemic and hypotensive drugs in blood by high-performance liquid phase-high resolution time-of-flight tandem mass spectrometry. Background technique [0002] Flight safety is a major issue related to the national economy and the people's livelihood. Hypertension and diabetes are common diseases among pilots. They are the basic diseases of many disabling diseases, and they are also important reasons for pilots to be grounded. Rational use of drugs is still an important measure for the treatment of hypertension and diabetes, so as to prevent heart, kidney, brain, nervous system and other complications caused by hypertension and diabetes, and prolong the flight life of pilots; however, antihypertensive drugs and hypoglycemic drugs Unreasonable use may lead to a sharp drop in blood pressure and blood sugar, resulting in sudden disability of the pilot. According to the "Civil Aviation Personnel Physical...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 王伟许海山李松林乔湜李清艳徐先发刘国如于红燕丁仁奎刘永锁
Owner 中国民用航空局民用航空医学中心
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