A method for detecting hypoglycemic and hypotensive drugs in blood by ultra-high performance liquid chromatography-mass spectrometry

Abstract

The invention provides a method for detecting 31 common blood pressure-lowering and blood sugar-lowering drugs in blood by an ultra high performance liquid-mass spectrometry, and is characterized in that the method comprises the following steps: (1) confirmation of detected target compounds; (2) sample processing: processing a sample by an organic solvent protein-deposition method; (3) primary screening detection: carrying out primary screening detection by a multiple reaction monitoring (MRM) mode of the ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) in different periods of time; (4) reexamining of the sample which is positive by the primary screening detection in the step (3): after treating and concentrating the sample which is positive by the screening detection, carrying out UPLC-MS / MS detection; and optionally, (5) reexamining of the sample which is positive by the reexamining detection in the step (4): reexamining by a high performance liquid chromatography-high resolution time-of-flight tandem mass spectrometry (HPLC-QTOF). According to a fact that the drugs have different retention time in the set detection conditions, a method for sectional detection is creatively completed. The required time for totally completing the detection of 31 drugs can be within 11 minutes.

Description

technical field [0001] The invention relates to a method for detecting hypoglycemic and hypotensive drugs in blood by ultra-high performance liquid phase-mass spectrometry. Background technique [0002] Flight safety is a major issue related to the national economy and the people's livelihood. Hypertension and diabetes are common diseases among pilots. They are the basic diseases of many disabling diseases, and they are also important reasons for pilots to be grounded. Rational use of drugs is still an important measure for the treatment of hypertension and diabetes, so as to prevent heart, kidney, brain, nervous system and other complications caused by hypertension and diabetes, and prolong the flight life of pilots; however, antihypertensive drugs and hypoglycemic drugs Unreasonable use may lead to a sharp drop in blood pressure and blood sugar, resulting in sudden disability of the pilot. According to the "Civil Aviation Personnel Physical Examination Certificate Managem...

AI Technical Summary

Problems solved by technology

Although document 6 also uses ultra-high performance liquid chromatography-mass spectrometry, there are only two types of drugs to be detected, and there are few types, and the sample pretreatment uses solid-phase extraction, which is complicated and time-consuming.

[0006] There are different types of antihypertensive and hypoglycemic drugs commonly used at home and abroad, and the above-mentioned detection methods in the prior art cannot cover the types of drugs commonly used in China

In addition, due to the large number of test subjects (in addition to more than 30,000 active pilots, it is also used to recruit flight students, foreign pilots and military-to-civilian pilots)), the test time is limited (affecting the pilots to obtain medical certificates), and the number of biological samples is small. There are many target compounds to be detected, and the existing methods cannot meet the above requirements

[0007] At present, there is still a lack of fast, easy, sensitive, reliable, reproducible and accurate methods for detecting common blood pressure and blood sugar lowering drugs that can be used for pilots' medication conditions

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