InDel molecular marker co-separated with cucumber powdery mildew resistance main effect gene

Abstract

The invention provides an InDel molecular marker co-separated with cucumber powdery mildew resistance main effect gene, which is named as InDel-MLO1, and is composed of a nucleotide sequence fragment shown in a SEQ ID NO.1 and a nucleotide sequence fragment shown in a SEQ ID NO.2; wherein the nucleotide sequence fragment shown in the SEQ ID NO.1 is co-separated with the anti-disease gene, and the nucleotide sequence fragment shown in the SEQ ID NO.2 is co-separated with the anti-disease gene and susceptible gene. The InDel molecular marker has high stability, can be used for auxiliary screening of powdery mildew resistance individual plant at a cucumber seedling stage in a simple, rapid and high flux mode; According to the SEQ ID NO.5 and the SEQ ID NO.6, difference of resistance candidate gene DNA length is generated due to insertion/deletion mutation, and several pairs of codominance InDel markers can be designed as the powdery mildew resistance screened molecular marker. The InDel molecular marker establishes a base for auxiliary breeding of molecular marker of powdery mildew resistance, and can greatly accelerates the process of cucumber powdery mildew resistance molecule breeding.

Description

Technical field [0001] The invention relates to genetic engineering technology, in particular to an InDel molecular marker co-segregated with cucumber powdery mildew resistance major genes. Background technique [0002] Cucumber (Cucumis sativus L.) is an annual herbaceous creeping climbing plant in the Cucurbitaceae family. It is native to northern India in the southern foothills of the Himalayas. my country has a history of more than 2,000 years of cultivation and is the secondary origin center of cucumbers. Cucumber is one of the top ten important vegetable crops in the world and one of the main vegetable crops in my country. As an important vegetable crop, cucumber has always been valued by breeders. [0003] In recent years, with the continuous expansion of the facility cultivation area, it can be cultivated off-season, annual production, and cucumber output has increased significantly, meeting the market demand. However, due to changes in conditions from open field producti...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the difficult problem of cucumber powdery mildew resistance breeding, to provide a co-dominant InDel molecular marker developed based on cucumber powdery mildew resistance candidate gene and powdery mildew main effect gene / QTL co-segregation, and this marker The difference can be clearly displayed by agarose gel electrophoresis, which is convenient for molecular assisted breeding of powdery mildew resistance

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