Portable nucleic acid isothermal amplification instrument

Abstract

The invention provides a portable nucleic acid isothermal amplification instrument. Directed at the detection characteristics of animal epidemic diseases and based on an open source Arduino hardware system, the instrument adopts a programmable isothermal amplification technology, and comprehensively covers isothermal detection methods. While realizing the detection function, hardware and software can be upgraded according to needs, existing isothermal amplification technologies can be corrected and new detection methods can be developed. The isothermal amplification instrument provided by the invention takes a universal controller Arduino uno as the main control panel, employs an independently developed full-bridge control panel to control a Peltier device in terms of heating and cooling circulation on an aluminum block, thus realizing a constant temperature. According to the control process, after thermistor and 22-bit high precision A / D conversion, then the conversion result is sent to Arduino for processing, and on-off of a bridge arm can be controlled in real time by pulse according to the processing result. At the same time, a graphic software platform is researched and developed independently, and the temperature and reaction time can be controlled independently.

Description

Technical field: [0001] The invention relates to the detection fields of molecular biology, microbiology, medicine and genetics, and the specific technical fields include portable and constant temperature amplification. Background technique [0002] Gene amplification technology, namely polymerase chain reaction (polymerase chain reaction), referred to as PCR, is one of the most important technologies in life sciences. Molecular analysis and detection capabilities can detect single-molecule DNA or samples containing only 1 target DNA molecule per 100,000 cells, so this method is widely used in molecular biology, microbiology, medicine, genetics and other fields. Rapid development. Due to the advantages of high sensitivity, strong specificity, rapidity and simplicity, PCR has shown great application value and broad development prospects in the field of pathogenic microbiology. [0003] However, the conventional PCR technology has the following disadvantages: (1) expensive P...

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Problems solved by technology

[0003] However, the conventional PCR technology has the following disadvantages: (1) expensive PCR equipment is required; (2) multiple factors affect the amplification effect; (3) often causes non-specific amplification; (4) the amplification reaction time is long, generally requiring several hours, it is difficult to promote the application at the grassroots level, etc.

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