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Stable lipase kit

A lipase and kit technology, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of inconvenient storage, unstable quality and high cost of reagents.

Active Publication Date: 2014-12-17
NINGBO RUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the existing reagents are not easy to store, and are prone to hydrolysis and demulsification, and precipitation will occur after long-term storage, the quality is unstable, it is not easy to store, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]

[0042] In the LPS test, the correlation experiment of embodiment 1 of the present invention and Roche reagent test:

[0043] sample number

[0044] 5

[0045] Stability of precipitation: when the reagent of Example 1 of the present invention and the control reagent (commercially available such as Roche reagent) are stored at 2-8°C for 12 months, the results of the precipitation content observation in the two reagents are observed.

[0046] time

[0047] Stability and sensitivity changes: when the reagent of Example 1 of the present invention and the control reagent (commercially available such as Roche reagent) were stored at 2-8°C for 12 months, the sensitivity of the two reagents was observed.

[0048] time

[0049] To sum up, it can be seen that the reagents of this example have accurate response and high sensitivity to the determination of LPS. At the same time, it effectively inhibits the attenuation of the active comp...

Embodiment 2

[0051]

Embodiment 3

[0053]

[0054]

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Abstract

The invention discloses a stable lipase kit which comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from 10-200mmol / L of buffer solution, 0.05-5g / L of sodium deoxycholate, 0.05-10g / L of sodium deoxycholate, 0.1-5mg / L of colipase, 1-20mmol / L of calcium chloride, 0.01-1% of preservative and 0.01-1% of surfactant; the reagent R2 is prepared from 10-200mmol / L of tartaric acid, 0.1-10g / L of taurodeoxycholic acid sodium salt, 0.01-1% of preservative, 0.01-2% of cosolvent, 0.01-2% of emulsifying agent, 0.01-2% of stabilizer and 0.1-0.5mmol / L of 6-methyl resorufin. The kit is high in sensitivity, small in error, stable in quality and convenient to store and has a strong function of protecting a substrate so as to improve the stability of the reagents to obtain a good detection effect. Thus, the kit has a high clinical application value.

Description

technical field [0001] The invention relates to a biological detection reagent, in particular to a stable lipase kit. [0002] Examples illustrate the meanings of some symbols in this application: [0003] Preservative 0.01-1% means adding 0.01-1g of preservative per 100ml of solution. [0004] The similarities in this application all represent such meanings, unless otherwise stated. Background technique [0005] Lipase (Lipase EC3.1.1.3., LPS, glyceride hydrolase) is a class of enzymes that hydrolyze oil and ester. The hydrolysis substrate of lipase is generally natural oil, and its hydrolysis site is the ester bond connecting fatty acid and glycerol in oil. It is different from other hydrolases. The lipase catalytic system is a heterogeneous system. The water-soluble enzyme catalysis occurs at the interface between the water-insoluble substrate and water. The catalytic mechanism of this interface is not very clear. The reaction mechanism between enzyme and substrate ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 谢蒙张闻周海滨王建飞
Owner NINGBO RUI BIO TECH
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