Method for extracting hyoscyamine from mandragora flower

A technology of datura flower and scopolamine, which is applied in the direction of organic chemistry and the like, can solve the problems of large amount of solvent, long extraction time, low extraction rate and the like, and achieves the effects of low production cost, improved extraction rate and improved solubility

Inactive Publication Date: 2014-12-24
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have many disadvantages, such as long extraction time, high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1

[0017] Add 1000g of Datura flower to 1 times 70% ethanol solution, pulverize with plant tissue crushing extraction method for 2 minutes, take it out, add 8 times 70% ethanol, adjust the pH to 4.5 with hydrochloric acid, and place it under ultrasound Extract in the extractor for 30 minutes, the ultrasonic power is 600W, the extract is centrifuged, the supernatant is added to the HZ-818 macroporous resin column for adsorption, the resin column is washed with water, and then 8 times the ammonia-containing 5% ammonia ethanol solution is added for elution For the active ingredients, the eluate is collected, concentrated to obtain the total alkaloids, and ethanol is recrystallized twice to obtain scopolamine with a content of 85.4%.

Example Embodiment

[0018] Example 2

[0019] Add 1000g of Datura flower to 3 times 75% ethanol solution, pulverize with plant tissue crushing extraction method for 1.5min, take it out, add 10 times 75% ethanol, adjust the pH to 5.0 with hydrochloric acid, and place Extract in an ultrasonic extractor for 40 minutes, the ultrasonic power is 800W, the extract is centrifuged, the supernatant is added to the D-72 macroporous resin column for adsorption, the resin column is washed with water, and then 10 times the ammonia ethanol solution with 7% ammonia is added. The effective components are removed, the eluate is collected, concentrated to obtain total alkaloids, and ethanol is recrystallized twice to obtain scopolamine with a content of 88.1%.

Example Embodiment

[0020] Example 3

[0021] Add 1000g of Datura flower to 2 times 80% ethanol solution, pulverize with plant tissue crushing extraction method for 1 min, take it out, add 9 times 80% ethanol, adjust the PH value of acetic acid to 5.5, and place it under ultrasound Extract in the extractor for 50 minutes, the ultrasonic power is 1000W, the extract is centrifuged, the supernatant is added to the D-101 macroporous resin column for adsorption, the resin column is washed with water, and then 9 times the ammonia-containing 10% ammonia ethanol solution is added for elution The active ingredients are collected and the eluate is concentrated to obtain total alkaloids, and ethanol is recrystallized twice to obtain scopolamine with a content of 86.5%.

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Abstract

The invention discloses a method for extracting hyoscyamine from mandragora flower, and belongs to the chemical fields of natural products, in particular to a combined method of smashing of plant tissue breaking process and ultrasonic extraction. The method comprises the following steps of: adding mandragora flower into 70-80% ethanol solution of which the weight is 1-3 times of that of the mandragora flower; smashing the mixture by means of plant tissue breaking extraction for 1-2 minutes; taking out the mandragora flower and adding 70-80% ethanol of which the weight is 8-10 times of that of the original weight; regulating PH value to be between 4.5 and 5.5; putting the mandragora flower into an ultrasonic extractor for extracting for 30-50 minutes; performing centrifugation; adding supernate into a macroporous resin column for performing adsorption; rinsing the resin column with water; adding 8-10 times of ammonia ethanol solvent elution effective components into the resin column; collecting and condensing eluant to obtain total alkaloids; performing ethanol recrystallization for twice to obtain high-purity hyoscyamine. The method has the advantages of easy operation, low energy consumption, mild reaction condition, low extraction cost and the like, and therefore, the method is a simple and easily-industrialized technique for producing hyoscyamine.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and relates to a method for extracting scopolamine from datura flowers, in particular to a method combining plant tissue crushing with ultrasonic extraction. Background technique [0002] Datura, also known as dog walnut, drunken flower, etc., belongs to Solanaceae and Datura is an annual herb. Datura flower can be used not only for anesthesia, but also for treating diseases, known as "Oriental anesthetic". Clinically, it has the effect of anesthesia and analgesia, can relieve visceral colic, and treat A-Synth syndrome caused by yellow antimony. It is an important traditional Chinese medicine commonly used. Hyoscyamine is an alkaloid isolated from Datura datura leaves, flowers, roots and seeds, with a content of 0.2% to 1.5%. It is a fine needle crystal and is easily soluble in general organic solvents. [0003] At present, the conventional methods for extracting scopolamine in China ar...

Claims

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Application Information

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IPC IPC(8): C07D451/10
CPCC07D451/10
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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