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Method for extracting hyoscyamine from mandragora flower

A technology of datura flower and scopolamine, which is applied in the direction of organic chemistry and the like, can solve the problems of large amount of solvent, long extraction time, low extraction rate and the like, and achieves the effects of low production cost, improved extraction rate and improved solubility

Inactive Publication Date: 2014-12-24
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have many disadvantages, such as long extraction time, high energy consumption, large amount of solvent used, low extraction rate, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 1000g Datura flower to 1 times of 70% ethanol solution, crush it with the plant tissue crushing extraction method, take it out for 2 minutes, then add 8 times of 70% ethanol, adjust the pH value to 4.5 with hydrochloric acid, place in ultrasonic Extract in the extractor for 30min, the ultrasonic power is 600W, the extract is centrifuged, and the supernatant is added to the HZ-818 macroporous resin column for adsorption. The active ingredient was collected and the eluate was concentrated to obtain the total alkaloids, and recrystallized twice from ethanol to obtain hyoscyamine with a content of 85.4%.

Embodiment 2

[0019] Add 1000g Datura flower into 3 times of 75% ethanol solution, crush it with plant tissue crushing extraction method, take it out for 1.5min, then add 10 times of 75% ethanol, hydrochloric acid to adjust the pH value to 5.0, place in Extract in an ultrasonic extractor for 40 minutes, the ultrasonic power is 800W, the extract is centrifuged, and the supernatant is added to a D-72 macroporous resin column for adsorption. Remove the active ingredient, collect the eluate, concentrate to obtain the total alkaloids, and recrystallize twice from ethanol to obtain hyoscyamine with a content of 88.1%.

Embodiment 3

[0021] Add 1000g Datura flower into 2 times of 80% ethanol solution, crush it with the plant tissue crushing extraction method, take it out for 1min, then add 9 times of 80% ethanol, adjust the pH value to 5.5 with acetic acid, place in ultrasonic Extract in the extractor for 50 minutes, the ultrasonic power is 1000W, the extract is centrifuged, the supernatant is added to the D-101 macroporous resin column for adsorption, the resin column is washed with water first, and then 9 times the ammonia water ethanol solution containing 10% ammonia is added to elute The active ingredient was collected and the eluate was concentrated to obtain the total alkaloids, and recrystallized twice from ethanol to obtain hyoscyamine with a content of 86.5%.

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Abstract

The invention discloses a method for extracting hyoscyamine from mandragora flower, and belongs to the chemical fields of natural products, in particular to a combined method of smashing of plant tissue breaking process and ultrasonic extraction. The method comprises the following steps of: adding mandragora flower into 70-80% ethanol solution of which the weight is 1-3 times of that of the mandragora flower; smashing the mixture by means of plant tissue breaking extraction for 1-2 minutes; taking out the mandragora flower and adding 70-80% ethanol of which the weight is 8-10 times of that of the original weight; regulating PH value to be between 4.5 and 5.5; putting the mandragora flower into an ultrasonic extractor for extracting for 30-50 minutes; performing centrifugation; adding supernate into a macroporous resin column for performing adsorption; rinsing the resin column with water; adding 8-10 times of ammonia ethanol solvent elution effective components into the resin column; collecting and condensing eluant to obtain total alkaloids; performing ethanol recrystallization for twice to obtain high-purity hyoscyamine. The method has the advantages of easy operation, low energy consumption, mild reaction condition, low extraction cost and the like, and therefore, the method is a simple and easily-industrialized technique for producing hyoscyamine.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and relates to a method for extracting scopolamine from datura flowers, in particular to a method combining plant tissue crushing with ultrasonic extraction. Background technique [0002] Datura, also known as dog walnut, drunken flower, etc., belongs to Solanaceae and Datura is an annual herb. Datura flower can be used not only for anesthesia, but also for treating diseases, known as "Oriental anesthetic". Clinically, it has the effect of anesthesia and analgesia, can relieve visceral colic, and treat A-Synth syndrome caused by yellow antimony. It is an important traditional Chinese medicine commonly used. Hyoscyamine is an alkaloid isolated from Datura datura leaves, flowers, roots and seeds, with a content of 0.2% to 1.5%. It is a fine needle crystal and is easily soluble in general organic solvents. [0003] At present, the conventional methods for extracting scopolamine in China ar...

Claims

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Application Information

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IPC IPC(8): C07D451/10
CPCC07D451/10
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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