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Cell separation method based on laser array coding and photo-induction

A laser array and separation method technology, applied in the field of biomedicine, can solve problems such as damage and hysteresis, and achieve the effects of reducing labor intensity and reducing the possibility of experimental operation errors

Active Publication Date: 2015-01-07
ZHONGBEI UNIV
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  • Abstract
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Problems solved by technology

However, the hysteresis and damage of the Joule heat induction method of the traditional resistance furnace urgently requires the exploration of a cell induction method with fast response speed, strong protection for cell activity, and conducive to automatic control.

Method used

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  • Cell separation method based on laser array coding and photo-induction
  • Cell separation method based on laser array coding and photo-induction
  • Cell separation method based on laser array coding and photo-induction

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Embodiment Construction

[0032] Such as figure 1 As shown, a laser array encoding and light-induced cell separation device includes a host computer 1, a repeater 2, a laser generator 3, a laser beam splitter 4, an optical switch 5, a high-speed CCD imaging system 9, and is used for cell manipulation. The microfluidic chip 7, the flushing liquid distribution equipment 11, the waste liquid treatment equipment 8 and the intelligent microscope platform 6, the host computer 1, the repeater 2, the laser generator 3, the laser beam splitter 4, and the optical switch 5 are connected in turn, the optical switch 5 is provided with an intelligent microscopic platform 6, and the intelligent microscopic platform 6 is provided with a microfluidic chip 7; A high-speed CCD imaging system 9 is provided on the upper side of the microfluidic chip 7 , and the high-speed CCD imaging system 9 is connected to the host computer 1 .

[0033]Among them, the upper computer: its manifestation is a computer and corresponding sup...

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Abstract

The invention relates to a cell separation technology, belongs to the field of biomedicine, and specifically relates to a cell separation method based on laser array coding and photo-induced micro-bubble precipitation. In prior art, in the fields like biosensor, mankind functional genome carrier research, rare cell screening, judicial evidence obtaining of sex crimes, and so on, the target cells for rapid separation and detection comprise too much information, the provided method solves the problem mentioned above. The matured image identifying technology and automatic control technology are effectively combined in the method, and thus the technologies are not just confined in single micro fluidic array or applied to single purpose.

Description

technical field [0001] The invention relates to cell separation technology in the field of biomedicine, in particular to a cell separation method based on laser array coding and light-induced microbubble separation. Background technique [0002] At this stage, the in situ visualized non-destructive operation of single cells urgently requires researchers to study novel cell micromanipulation mechanisms from the single cell level. As an important part of modern biology, cell screening is also one of the key steps in cell research. Through high-throughput precise screening, a large number of target cells can be obtained, which can be applied to the research of cell biology, biomedicine, tissue engineering, pharmacokinetics, combinatorial chemistry and judicial identification. These pathological or physiological behaviors are to a large extent regulated by the microenvironment of the cells, and the microenvironment of the cells is relatively complex, such as the biochemical and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/34C12M1/00
CPCC12M23/16C12M41/48
Inventor 谭秋林孙东张晓飞张文栋熊继军刘俊薛晨阳王晓龙张洋罗涛李超
Owner ZHONGBEI UNIV
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