Multiplex PCR diagnosis method capable of quickly distinguishing porcine circovirus 1 type (PCV1) and 2 type (PCV2) and special-purpose kit

Abstract

The invention patent relates to a diagnosis technology in the technical field of agriculture. Specific primers L1, L2 and L3 are designed automatically, and virus DNAs are extracted; on the basis, the PCR method is used for discriminating and detecting different serotypes of the porcine circovirus, the size of an estimated amplified fragment of the amplified porcine circovirus II type (PCV2) is 404bp, and the size of an estimated amplified fragment of the amplified porcine circovirus I type (PCV1) is 198bp; on the basis, a special-purpose kit is successfully developed. Compared with the prior art, the diagnosis method and the special-purpose kit have stronger specificity and sensibility, and compared with methods of virus separation, IFA and the like, the coincidence rate of the method can reach more than 90%. The amplified results of the primers for a porcine reproductive and respiratory syndrome virus, a hog cholera virus, a porcine pseudorabies virus, porcine parvovirus are all feminine, and the primers can be widely used for detecting the porcine circovirus in clinical applications and laboratories.

Description

technical field [0001] The patent of the present invention relates to diagnostic technology in the field of veterinary biotechnology, specifically a multiplex PCR diagnostic method and a special kit capable of quickly distinguishing porcine circovirus type 1 (PCV1) and type 2 (PCV2). Background technique [0002] Porcine circovirus (PCV) is a small, icosahedral symmetric, non-enveloped, single-stranded circular DNA virus. The diameter of the virus particle is about 17nm, which is one of the animal viruses with the smallest DNA found so far. Circoviruses have two genotypes: PCV1, which is nonpathogenic, and PCV2, which is pathogenic. Porcine circovirus type 2 is also the main pathogen that causes multisystemic weakness syndrome (PMWS) in weaned piglets. It brings huge economic losses to the breeding industry and is one of the important diseases that endanger the world's pig industry at present. Although porcine circovirus type 1 is not pathogenic, it often contaminates pig...

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Problems solved by technology

[0003] The purpose of the present invention is to aim at the current situation that PCV2 infection is ubiquitous in my country, which brings serious economic losses to the pig industry, and PCV1 is often polluted in the culture process of PCV2, and provides a method that can facilitate the laboratory to carry out cell culture and PCV2 cytotoxic purification. Diagnostic methods and dedicated kits for on-the-go monitoring

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