Acute brain and spinal ischemia/reperfusion injury cellular network model as well as construction method and application thereof

A technology of reperfusion injury and cell network, applied in the field of cell network model and construction of acute ischemia/reperfusion injury of the brain and spinal cord, which can solve the problems of low degree of tight connection and unsatisfactory
CN104312969AInactive Publication Date: 2015-01-28DALIAN MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN MEDICAL UNIVERSITY
Publication Date
2015-01-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of biological medicines and in particular relates to an acute brain and spinal ischemia / reperfusion injury cellular network model as well as a construction method and application thereof. The cellular network model comprises primary isogenous central nervous system capillary endothelial cells, astrocytes and neurons, wherein the endothelial cells, astrocytes and neurons are three-dimensionally co-cultured according to the relative spatial position and quantity relations of the endothelial cells, astrocytes and neurons in vivo; the environment of oxygen and glucose deprivation / reoxygenation and recovery of glucose in a central nervous system during acute brain / spinal ischemia / reperfusion injury in vivo is simulated by utilizing a low oxygen regulation system / ordinary cell culture system and glucose-free cell culture fluid / normal cell culture fluid. The cellular network model has the beneficial effects that a medicine research mode of adopting the central nervous system capillary endothelial cell culture fluid in lumens of inserts for administration is put forward for the first time, so that the cellular network model further conforms to the organism pharmacokinetics; the cellular network model is used for further research of acute brain / spinal ischemia / reperfusion injury emergence and development mechanisms; the new cellular network model is provided for research and evaluation of clinical diagnostic markers and medicines.
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Description

Technical field

[0001] The present invention belongs to the field of biomedicine technology, and particularly relates to a primary generation formed by central nervous system capillary endothelial cells (E), astrocytes (astrocytes, A) and neurons (neurons, N) A cell network model of brain and spinal cord acute ischemia / reperfusion injury based on a homologous three-cell four-dimensional model and its construction method and application. Background technique

[0002] Acute ischemic stroke of the brain and spinal cord has the characteristics of high morbidity, high mortality, high disability and high recurrence rate. It is a major medical problem that seriously endangers human life and health. Clinically, acute drug delivery after the onset of the disease and preventive drug delivery to high-risk groups have been used to intervene in acute ischemic stroke of the brain and spinal cord, but with little effect.

[0003] In recent years, there have been more and more researches on acute...

Claims

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