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Preservation method of empoasca flavescens living bodies

A technology of a small green leafhopper and a preservation method, which is applied in the preservation of human or animal bodies, botanical equipment and methods, horticulture, etc., can solve the problems of difficult collection, high mortality in long-distance transportation, and achieve long and repeatable preservation periods. The effect of strong sex and high survival rate

Inactive Publication Date: 2015-02-04
信阳市农业科学院
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies in the prior art, thereby providing a method for preserving the living body of the green leafhopper adults and nymphs, so as to solve the difficulty and distance of collecting insects with smaller individuals like the green leafhopper in the prior art. The problem of excessive mortality during distance transportation or carry storage

Method used

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Embodiment Construction

[0043] In order to make the content of the present invention easier to understand, the technical solution of the present invention will be further described below in conjunction with specific embodiments.

[0044] Embodiments of the present invention provide a method for long-distance transportation or storage of green leafhopper adults and nymphs, comprising the following steps:

[0045] Seedbed preparation: Start site preparation 30 days before the collection of the small green leafhopper. The matrix of the kenaf seedbed is composed of hemp field soil and peat ash. The site preparation should be flat, empty at the top and solid at the bottom, with no rubbish, moist soil, and the local surface layer down 3 cm. Just water in time when the soil layer is dry, wherein, the ratio of described kenaf seedbed matrix is ​​hemp field soil: peat ash=4:1;

[0046] Seed germination: 2 days before sowing, soak the seeds of kenaf variety Zahong 952 in 28°C water for 24 hours, then pour off ...

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Abstract

The invention discloses a preservation method of empoasca flavescens living bodies. The preservation method comprises the following steps: providing empoasca flavescens fresh feeds and empoasca flavescens; preserving the empoasca flavescens with the quantity of N and the empoasca flavescens fresh feeds with the mass of M grams into a living body preservation container with the volume of V cubic centimeters, wherein the relation between the mass M grams of the empoasca flavescens fresh feeds and the quantity N of the empoasca flavescens at least meets the following fitting relational expression: M=0.0326V+0.066 and N=0.3339V+3.7274; the N is a round-off natural number of a calculation value of (0.3339V+3.7274). The preservation method of the empoasca flavescens living bodies has the following advantages that the operation is simple and convenient and the repeatability is strong; the preservation period of test empoasca flavescens is long and the test empoasca flavescens can be preserved for 4-8 days in a test tube; the survival rate is high and reaches 90%; the received test empoasca flavescens can be subjected to subculture feeding in different places.

Description

technical field [0001] The invention relates to a living body preservation method of small-scale leafhopper insects, in particular to a living body preservation method for adults and nymphs of the green leafhopper in Matian. Background technique [0002] Small green leafhoppers belong to the order Hemiptera and belong to the family Cicadellidae, commonly known as floating dust, leaf collembola, etc. Leafhoppers have a wide range of hosts, and the main damage crops are wheat, cotton, rice, hemp, mallow, sesame, peanuts, beans, tobacco, tea, eggplant, potatoes, etc. Kenaf ( Hibiscus cannabinus ), also known as kenaf and hibiscus, is an annual herbaceous crop of Malvaceae and Hibiscus. Pharmaceutical and many other industries. In recent years, with the change of climate, the leafhopper of hemp field has gradually risen to be the main pest. There are two species of leafhopper of kenaf field, which are the small green leafhopper of Banjing ( Empoas casakaii Dworakowska) and...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01N1/02A01G1/00
CPCA01G22/00A01K67/033A01N1/02
Inventor 乔利潘兹亮吕玉虎张丽霞郭晓彦李梅
Owner 信阳市农业科学院
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