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The function and application of blood shear stress response protein 1 in the treatment of atherosclerosis

A technology for atherosclerosis and purpose, applied in the field of gene function and application, can solve the unexplained problems of biological function and possible mechanism of action, and achieve the effect of promoting atherosclerosis

Active Publication Date: 2017-01-04
武汉惠康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above research basis, we believe that RECS1 may play an important role in the characteristic pathophysiological process of atherosclerosis, but its biological function and possible role in the characteristic pathophysiological process of atherosclerosis mechanism has not been elucidated

Method used

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  • The function and application of blood shear stress response protein 1 in the treatment of atherosclerosis
  • The function and application of blood shear stress response protein 1 in the treatment of atherosclerosis
  • The function and application of blood shear stress response protein 1 in the treatment of atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Obtaining a mouse atherosclerosis model (AS)

[0044] 1. Grouping of experimental animals: 8-week-old, weighing 19-25g, male, ApoE - / - Mice and RECS1 - / - ApoE - / - Mice were fed with high-fat diet (Western Diets, HFD) and low-fat diet (Normal chow, NC), respectively, ApoE - / - HFD group, ApoE - / - NC Group, RECS1 - / - ApoE - / - HFD group, RECS1 - / - ApoE - / - There were 4 groups in the NC group, with 20 animals in each group.

[0045] 2. The atherosclerosis model is induced by high-fat diet. The operation process:

[0046] Using ApoE - / - Mice and RECS1 - / - ApoE - / - In mice, an AS model was established, and phenotype correlation analysis was performed to clarify the role of RECS1 gene on atherosclerotic disease. From 8 weeks old, the mice in the HFD group were sacrificed and the samples were collected after being fed with the high-fat diet for 28 weeks, and the mice in the NC group were sacrificed and the samples were fed with the whole low-fat diet for 2...

Embodiment 2

[0047] Example 2 Determination of plaque area in AS model mice

[0048] 1. Mice terminal tissue harvesting

[0049] Mice were fed with high-fat or low-fat diet until 28 weeks, weighed, anesthetized the mice with 3% sodium pentobarbital, 90 mg / kg, fixed them on the sampling plate with a needle, and wet the skin of the chest and abdomen of the mice with gauze. Cut the chest cavity with ophthalmic scissors, expose the heart, cut open the right atrial appendage, insert the needle of the infusion set into the left ventricle, and slowly inject 10-15 mL of PBS buffer with a 50 mL syringe. Continue to inject 10-15mL of formaldehyde. After the perfusion, the thoracic and abdominal organs were removed, and only the heart was preserved. The mice were placed under a microscope, the fascia and adipose tissue around the aortic arch were separated, the heart was cut at the beginning of the ascending aorta, the middle of the thoracic aorta, and about 3 mm below the common neck and clavicle ...

Embodiment 3

[0062] Example 3 Determination of plaque stability in AS model mice

[0063] 1. Determination of the area size of the necrotic center of the aortic sinus

[0064] Hematoxylin and eosin staining (HE staining), the method was the same as that of Example 2.4, and the tissue containing cholesterol crystals and no nucleus fibrous structure was selected to take pictures under a microscope.

[0065] Measurement of the area of ​​the necrotic center: The area of ​​the necrotic center was circled using Image-Pro Plus 6.0 image analysis software.

[0066] 2. Determination of collagen content in aortic sinus:

[0067] Sirius red (PSR) staining, the main steps are: take aortic sinus paraffin white slices and bake at 55℃ for 30min→xylene for 2min, 3 times→100% alcohol for 1min→95% alcohol for 1min→70% alcohol for 1min→rinse with running water for 10min→ Double-distilled water for 1min→0.2% phosphomolybdic acid for 2min→0.1% Sirius scarlet picric acid solution was dropped on the tissue, st...

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Abstract

The invention discloses the function and application of blood shear stress response protein 1 (RECS1) in treating atherosclerosis, belonging to the field of gene function and application. The present invention takes ApoE‑ / ‑ mice and RECS1‑ / ‑ApoE‑ / ‑ mice as experimental objects, and obtains atherosclerosis models through high-fat diet induction. The results show that compared with ApoE‑ / ‑ mice, RECS1 gene defects It significantly reduces the plaque area of ​​the aorta, enhances the stability of the plaque in the aortic sinus, and significantly reduces the inflammatory response. This indicates that the function of RECS1 in atherosclerosis is mainly reflected in the promotion of aortic plaque formation, especially atherosclerosis. According to the function of RECS1, RECS1 can be used as a drug target for screening drugs for preventing, alleviating and / or treating atherosclerosis, and the inhibitor of RECS1 can be used for preparing drugs for preventing, alleviating and / or treating atherosclerosis.

Description

technical field [0001] The invention belongs to the field of function and application of genes, and in particular relates to the function and application of a blood shear stress response protein 1 (RECS1) in the treatment of atherosclerosis, in particular to the preparation of RECS1 for preventing, relieving and / or treating atherosclerosis sclerosis-like drug application. Background technique [0002] Cardiovascular and cerebrovascular diseases are the main cause of death in many developed countries, and the morbidity and mortality in my country are also increasing year by year. The basis of cardiovascular and cerebrovascular diseases is atherosclerosis (Atheosclersis, AS). Atherosclerosis can thicken, harden and narrow the arterial wall, leading to many cardiovascular and cerebrovascular events. The rupture of atherosclerotic unstable plaque, platelet aggregation and acute coronary stenosis and occlusion caused by thrombosis are important causes of acute coronary syndrome ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K48/00A61K39/395A61K45/00A61P9/10
Inventor 李红良赵光年王丕晓邓克穷
Owner 武汉惠康基因科技有限公司
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