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Function and application of II-type oncostatin M receptor in treating atherosclerosis

A technology of atherosclerosis and antibodies, which is applied in the relief and/or treatment of drugs for atherosclerosis. In the field of preparation and prevention, Osmr can solve problems such as biological functions and possible mechanisms of action that have not yet been elucidated, and achieve the goal of promoting Effects of atherosclerosis

Inactive Publication Date: 2015-01-07
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above research basis, we believe that OSMR may play an important role in the characteristic pathophysiological process of atherosclerosis, but its biological function and possible role in the characteristic pathophysiological process of atherosclerosis mechanism has not been elucidated

Method used

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  • Function and application of II-type oncostatin M receptor in treating atherosclerosis
  • Function and application of II-type oncostatin M receptor in treating atherosclerosis
  • Function and application of II-type oncostatin M receptor in treating atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Obtaining a mouse atherosclerosis model (AS)

[0046] 1. Grouping of experimental animals: 8-week-old, weighing 19-25g, male, ApoE - / - Mice and Osmr - / - ApoE - / - Mice were fed with high-fat diet (Western Diets, HFD) and low-fat diet (Normal chow, NC), respectively, ApoE - / - HFD group, ApoE - / - NC Group, Osmr - / - ApoE - / - HFD group, Osmr - / - ApoE - / - There were 4 groups in the NC group, with 20 animals in each group.

[0047] 2. The atherosclerosis model is induced by high-fat diet. The operation process:

[0048] Using ApoE - / - Mice and Osmr - / - ApoE - / - In mice, an AS model was established, and phenotype correlation analysis was performed to clarify the role of Osmr gene on atherosclerotic disease. From 8 weeks old, the mice in the HFD group were sacrificed and the samples were collected after being fed with the high-fat diet for 28 weeks, and the mice in the NC group were sacrificed and the samples were fed with the whole low-fat diet for...

Embodiment 2

[0049] Example 2 Determination of plaque area in AS model mice

[0050] 1. Mice terminal tissue harvesting

[0051] Mice were fed with high-fat or low-fat diet until 28 weeks, weighed, anesthetized the mice with 3% sodium pentobarbital, 90 mg / kg, fixed them on the sampling plate with a needle, and wet the skin of the chest and abdomen of the mice with gauze. Cut the chest cavity with ophthalmic scissors, expose the heart, cut open the right atrial appendage, insert the needle of the infusion set into the left ventricle, and slowly inject 10-15 mL of PBS buffer with a 50 mL syringe. Continue to inject 10-15mL of formaldehyde. After the perfusion, the thoracic and abdominal organs were removed, and only the heart was preserved. The mice were placed under a microscope, the fascia and adipose tissue around the aortic arch were separated, the brachiocephalic trunk was cut off, and placed in a 5 mL EP tube filled with 4% paraformaldehyde. The aorta was cut in the middle and about...

Embodiment 3

[0066] Example 3 Determination of plaque stability in AS model mice

[0067] 1. Determination of the area of ​​the necrotic center of the aortic sinus.

[0068] Hematoxylin and eosin staining (HE staining), the method was the same as that of Example 2.4, and the tissue containing cholesterol crystals and no nucleus fibrous structure was selected to take pictures under a microscope.

[0069] Measurement of the area of ​​the necrotic center: The area of ​​the necrotic center was circled using Image-Pro Plus 6.0 image analysis software.

[0070] 2. Determination of collagen content in aortic sinus:

[0071] Sirius red (PSR) staining, the main steps are: take aortic sinus paraffin white slices and bake at 55℃ for 30min→xylene for 2min, 3 times→100% alcohol for 1min→95% alcohol for 1min→70% alcohol for 1min→rinse with running water for 10min→ Double-distilled water for 1min→0.2% phosphomolybdic acid for 2min→0.1% Sirius scarlet picric acid solution was dropped on the tissue, stai...

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Abstract

The invention discloses a function and an application of an II-type oncostatin M receptor (Osmr) in treating atherosclerosis, belonging to the field of gene functions and applications. According to the function and the application disclosed by the invention, with ApoE<- / ->mice and Osmr<- / ->ApoE<- / ->mice as experimental subjects, an atherosclerosis model is obtained by virtue of high-fat diet induction, and a result shows that compared with the ApoE<- / ->mice, plaque area of aorta of the Osmr<- / ->ApoE<- / ->mice is remarkably reduced due to Osmr gene defect, the stability of aortic sinus plaque is strengthened, inflammatory response is remarkably alleviated, and the result shows that the functions of the Osmr in atherosclerosis mainly embody in promoting plaque of the aorta to form, especially promoting the atherosclerosis. Aiming at the functions of the Osmr, the Osmr can serve as a drug target used for screening drugs for preventing, alleviating and / or treating the atherosclerosis, and an inhibitor of the Osmr can be used for preparing the drugs for preventing, alleviating and / or treating the atherosclerosis.

Description

[0001] technical field [0002] The invention belongs to the field of gene function and application, in particular to the function and application of a type II oncostatin M receptor (Osmr) in the treatment of atherosclerosis, in particular to the preparation of Osmr for preventing, relieving and / or treating atherosclerosis Atherosclerosis drug application. Background technique [0003] Cardiovascular and cerebrovascular diseases are the main cause of death in many developed countries, and the morbidity and mortality in my country are also increasing year by year. The basis of cardiovascular and cerebrovascular diseases is atherosclerosis (Atheosclersis, AS). Atherosclerosis can thicken, harden and narrow the arterial wall, leading to many cardiovascular and cerebrovascular events. The rupture of atherosclerotic unstable plaque, platelet aggregation and acute coronary stenosis and occlusion caused by thrombosis are important causes of acute coronary syndrome (ACS). [0004]...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K48/00A61K39/395A61P9/10
Inventor 李红良郭森黄玲向梅张鹏
Owner WUHAN UNIV
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