Tuning fork carotid bifurcate flow cavity and circulating system device

Abstract

The invention discloses a tuning fork carotid bifurcate flow cavity and circulating system device in the technical field of biological engineering mechanics. The device consists of a flow cavity, a nutrient solution circulating system and a throttle valve, wherein the inlet of the flow cavity is connected with the nutrient solution circulating system; two outlets of the flow cavity are connected with the nutrient solution circulating system through the throttle valve respectively; and a cell culture window is embedded into the outside wall of the carotid sinus root of the flow cavity. Three-dimensional disturbance flow is realized on the cell culture window for simulating the wall shape of the outside disturbance area of the real carotid sinus root; the cell culture window is convenient to assemble and disassemble; and biological response of cultured cells to an approximately real arterial disturbance area environment can be quantitatively observed by using the flow cavity and the circulating system so as to provide a new tool for promoting atherosclerosis mechanical and biological research.

Description

technical field [0001] The invention relates to a device in the technical field of biomechanics, in particular to a tuning fork type human carotid bifurcation (TF-AHCB) flow chamber and circulation system device. Background technique [0002] Clinical observation and autopsy found that the human carotid bifurcation is a typical atherosclerotic lesion. In the study of atherosclerotic lesions, the disturbed area of ​​the lateral wall of the root of the carotid sinus is the most studied site. [0003] Biomechanical studies have confirmed that the flow in atherosclerotic lesions is a disturbed flow characterized by low shear stress distribution. Mechanobiological studies have shown that low shear stress disturbed flow may cause changes in active substances such as cell membrane receptors, signaling protein kinases, cytokines, and extracellular matrix, resulting in vascular reconstruction activities such as cell dedifferentiation, migration, hypertrophy, proliferation, and apopt...

AI Technical Summary

Problems solved by technology

The two improved flow chambers can form a disturbed flow on the cell culture area, but the disadvantage is that the disturbed flow of the two is two-dimensional, which is fundamentally different from the three-dimensional flow in the disturbed area of ​​the real artery

The above three flow chambers also have a common disadvantage: the entire flow chamber must be disassembled to install and obtain the culture cell window, which is time-consuming and labor-intensive

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