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Zygosaccharomyces rouxii strain for producing D-arabitol and application thereof

A technology for yeast strains and fermentation broth, applied in fermentation, fungi, microorganism-based methods, etc., can solve problems such as high equipment requirements, serious environmental pollution, and complex processes, and achieve the effect of technical route promotion.

Inactive Publication Date: 2015-02-11
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical synthesis method is obtained by catalytic reduction of D-arabitol or D-lyxose. This method has complex processes, high equipment requirements, serious environmental pollution, and large-scale production has not yet been realized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The specific operation process is as follows:

[0024] 1. Hyperosmotic enrichment culture

[0025] Prepare enriched medium: 40% D-glucose, 1% yeast extract, 1% peptone.

[0026] Weigh 1.0 g of different nectar samples respectively, add them into a 250ml Erlenmeyer flask containing 20ml enrichment medium, and vortex evenly. 30°C, 200r / min, shake flask culture for 3 days.

[0027] After the final enriched culture was serially diluted, 200 μl was pipetted and spread onto a hypertonic plate, and cultured at 30° C. for 3 days.

[0028] A single colony of suspected yeast that grew well was selected and purified by streaking, and the slant was stored in a refrigerator at 4°C.

[0029] 2. Preliminary screening by TLC method

[0030] Prepare fermentation medium: 20% D-glucose, 1% yeast extract, 1% peptone.

[0031] In the ultra-clean workbench, take a ring of each of the different yeast strains separated and put them into a 250ml Erlenmeyer flask containing 20ml of fermenta...

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Abstract

The invention discloses a zygosaccharomyces rouxii strain for producing D-arabitol and application thereof, and relates to the fields of development and application of a microorganism industrial strain. The hypertonic resistant yeast zygosaccharomyces rouxii for producing D-arabitol is separated from natural chaste honey. The strain disclosed by the invention is preserved in China Center For Type Culture Collection with preservation No. of CCTCC NO: M 2014205. 5L fermentation tank test tracking shows that the strain is normal in metabolism and stable in fermentation performance. The yeast strain is used for producing D-arabitol by converting substrate D-glucose, which is simple in process and capable of realizing conservation and environmental protection, and a conversion rate can reach 40%; the strain has practical application value, and is an excellent yeast strain for producing D-arabitol.

Description

technical field [0001] The invention relates to the field of development and application of microbial industrial strains, and relates to the screening of D-arabitol-producing yeast and a technical method for converting D-glucose to produce D-arabitol. [0002] Background technique [0003] D-arabitol is a naturally occurring five-carbon polysaccharide alcohol, also known as D-(+)-arabitol, D(+)-glutaritol, and its molecular formula is C 5 h 12 o 5 , and xylulose and xylitol are isomers. At room temperature, the pure D-arabitol product is a white crystal with a sweet taste and strong hygroscopicity. D-arabitol is a natural low-calorie sweet substance with high nutritional value, anti-caries, and its metabolism in the biological body does not affect insulin levels. It can be used as a nutritional and therapeutic agent for diabetics, and prevents dental caries for children. Food, food additive, but also an important synthetic intermediate of neurological drugs. Therefore,...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/18C12R1/645
CPCC12P7/18C12N1/145C12R2001/645
Inventor 齐向辉王旭林静朱静斐罗艳王亮孙文敬
Owner JIANGSU UNIV
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