MSTN gene molecular marker selection method for goat growth characters

A growth trait and molecular marker technology, applied in the field of molecular biology, can solve the problems of slow genetic progress and low accuracy, and achieve the effects of speeding up the breeding process, high selection accuracy, and reducing breeding costs.

Inactive Publication Date: 2015-02-11
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of low accuracy of growth trait phenotype selection and slow genetic progress in goat breeding, provide a method for selection of MSTN gene molecular markers for growth traits of goats, improve the accuracy of growth trait selection of Boer goats, and accelerate Breeding process of new breed of goat with Boer goat as breeding material

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  • MSTN gene molecular marker selection method for goat growth characters
  • MSTN gene molecular marker selection method for goat growth characters
  • MSTN gene molecular marker selection method for goat growth characters

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Embodiment 1

[0017] Embodiment 1, the MSTN gene molecular marker selection method of Boer goat growth traits

[0018] Materials and Methods:

[0019] (1) Phenotypic data of samples and growth traits

[0020] The phenotype data of 107 Boer goats and their growth traits were provided by Liuhe Animal Base of Jiangsu Academy of Agricultural Sciences. About 0.5g of ear tissue was collected from each individual, put into a 1.5ml Eppendoff tube, put in an ice box, and brought back to the laboratory- Freeze at 20°C. Phenotype data include birth weight, weight at six months, daily gain at six months, weight at nine months, daily gain at nine months, body height at birth, body height at six months, body height at nine months, bust at birth, and Data on growth traits such as age tube circumference.

[0021] (2) Method

[0022] 1) DNA was extracted by conventional phenol and chloroform methods, and DNA quality was detected by electrophoresis and OD260 / 280 determination.

[0023] 2) According to t...

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Abstract

The invention discloses an MSTN gene molecular marker selection method for goat growth characters. The method comprises the following steps: (1) designing a specific nucleic acid primer aiming at a 5'UTR region of a goat MSTN gene and carrying out PCR amplification on a to-be-detected goat genome DNA to obtain 593bp of amplification product; (2) carrying out enzyme digestion and electrophoresis analysis on the PCR product by using a DraI restriction enzyme to generate three genetypes, wherein AA type is 593bp; AB type is 593bp, 424bp and 174bp; and BB type is 424bp and 174bp; and (3) selecting AB type and BB type individual bodies to reserve seeds for planting because the weight and body size indexes of AB type and BB type individual bodies are obviously higher than those of the AA type individual body. According to the method, the selection accuracy of the boer goat growth characters can be improved, and the new variety breeding process of the goat taking the boer goat as a breeding material is quickened.

Description

technical field [0001] The invention relates to a detection method and application range of a polymorphic site in the 5'UTR region of goat myostatin gene (Myostatin, MSTN), belonging to the field of molecular biology. Background technique [0002] Growth is the main economic trait of meat goats, and it is also a quantitative trait. The genetic progress of phenotypic selection is slow, and the development of molecular markers for assisted selection can speed up the genetic progress. Myostatin gene (Myostatin, MSTN), also known as growth differentiation factor 8 (Growth Differenti-ation Factor 8, GDF-8), is a member of the transforming growth factor β (Transforming Growth Factor beta, TGF-β) superfamily. Mc Pherron et al. (1997) first found in mice that MSTN is an important gene affecting musculoskeletal muscle weight in animals. The normal function of MSTN gene is to regulate the total amount of animal skeletal muscle and keep it in proper proportion, which plays an importan...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/683C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2521/301
Inventor 孟春花曹少先傅泽红丰秀静张俊钱勇
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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