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Method for quickly and efficiently extracting baicalin

A technology of baicalin and scutellaria baicalensis is applied in the field of extraction of effective components of Chinese herbal medicine, and achieves the effects of less environmental pollution, good chemical stability and shortening of extraction time.

Active Publication Date: 2015-02-18
陕西笃诚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Imidazole-based ionic liquids refer to a class of ionic liquids with different cationic substituents containing imidazole groups. At present, there is no research report on the extraction of baicalin with imidazole-based ionic liquids.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Comparative experiment of saturated solubility of baicalin in 1-octyl-3-methylimidazolium bromide (IL) aqueous solution, water and 70% ethanol respectively.

[0021] Weigh 5 parts of baicalin 50mg and place them in 5mL centrifuge tubes respectively, add 2mL each of distilled water, 70% ethanol, 0.5M IL aqueous solution, 0.75M IL aqueous solution and 1M IL aqueous solution, seal and fix on a micro-oscillator for 2h, ultrasonic After 30min, the saturated solution was left to stand for 1h, and the supernatant saturated solution was taken for appropriate constant-volume dilution (the dissolved part of saturated distilled water was directly injected for analysis; take 0.5mL of 70% ethanol saturated solution, and dilute it in a 10mL volumetric flask with methanol; Draw 0.2mL of saturated solutions of 0.5M IL, 0.75M IL, and 1M IL, and use methanol to constant volume in a 50mL volumetric flask), and the diluted solution is subjected to HPLC content determination, and the measure...

Embodiment 2

[0025] Accurately weigh 1.0 g of Scutellaria baicalensis powder passed through a 40-mesh sieve into a chicken heart bottle, add 6 mL of an aqueous solution of 1-octyl-3-methylimidazolium bromide with a concentration of 1.0M, and microwave under the conditions of 400W microwave power and a temperature of 80°C Extract 90S, and obtain the extract containing baicalin through liquid-solid separation.

[0026] The measurement results show that: the extraction rate of baicalin in Scutellaria baicalensis extracted by the method of the present invention is 25.11% (the extraction rate is the ratio of the quality of baicalin in the extract to the quality of Scutellaria baicalensis used for extraction).

[0027] Compared with the Pharmacopoeia method:

[0028] Pharmacopoeia method: Accurately weigh 0.15 g of Scutellaria baicalensis powder passed through a 40-mesh sieve into a chicken heart bottle, add 20 mL of 70% ethanol, reflux in a water bath for 3 hours, and separate liquid-solid to o...

Embodiment 3

[0031] Accurately weigh 1.0 g of Scutellaria baicalensis powder passed through a 40-mesh sieve into a chicken heart bottle, add 16 mL of 1.0 M 1-octyl-3-methylimidazolium bromide aqueous solution, and extract under the conditions of microwave power 600W and temperature 80°C 120S, the extract containing baicalin was obtained by liquid-solid separation.

[0032] The measurement results show that: the extraction rate of baicalin in Scutellaria baicalensis extracted by the method of the present invention is 25.20%.

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Abstract

The invention provides a method for quickly and efficiently extracting baicalin. The method comprises the following steps: grinding scutellaria baicalensis, and extracting baicalin by ionic liquid, namely a 1-octyl-3-methylimidazole bromide aqueous solution, under the action of microwave enhancement. According to the method, baicalin is extracted by a non-volatile, noncombustible and nonexplosive green solvent, namely the ionic liquid; the extraction process is easy and convenient to operate and easy to perform, the extraction time can be greatly shortened, and the extraction efficiency can be improved. The method has application prospects on the aspects of extraction and analysis of baicalin.

Description

technical field [0001] The invention relates to a method for extracting effective components of Chinese herbal medicines, in particular to a method for rapidly and efficiently extracting baicalin from Scutellaria baicalensis by using a green solvent-ionic liquid. Background technique [0002] Scutellaria baicalensis Georgi is the dry root of Scutellaria baicalensis, which is widely distributed in nature and mainly produced in Hebei, Shanxi, Liaoning, Shaanxi, Shandong, Heilongjiang and other places. It is cold in nature and bitter in taste. It has the functions of clearing away heat and dampness, purging fire and detoxification, stopping bleeding, and anti-abortion. It is a commonly used traditional Chinese medicine. Baicalin is the main effective chemical component of Scutellaria baicalensis. It is widely used in Chinese patent medicine and veterinary medicine. It is also a good functional beauty cosmetic raw material [Gao Z, el al.Biochem Biophys Acta.1999,1472(3):643-650 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07Y02P20/54
Inventor 张立伟张琴赵三虎
Owner 陕西笃诚医药科技有限公司
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