A method for identifying salt tolerance of sweet sorghum

Abstract

The invention discloses an identification method for salt tolerance of sweet sorghum. The method comprises the following steps: preparing salt-containing culture solution and a salt-containing matrix; preparing a plurality of foam sheet germination beds, wherein a plurality of cavities with equal sizes and shapes are uniformly dug in each foam sheet, and holes are formed in the bottoms of the cavities; filling the cavities in the foam sheets with the salt-containing matrix, sowing sweet sorghum seeds, covering with mulching films, and placing the foam sheets in the same pool filled with the salt-containing culture solution for culture; performing salt tolerance screening and identification according to the seed germination rate of each foam sheet germination bed, the height of seedlings and fresh weight per plant. According to the identification method for the salt tolerance of the sweet sorghum, the salt tolerance in both a germination period and a seedling period of the sweet sorghum can be identified, the identification result is accurate, the identification error is small, and standards of proper salt concentration and different salt tolerance levels can be provided for large-scale screening of sweet sorghum genetic resources and selective breeding of the salt-tolerant sweet sorghum variety; the identification efficiency is high and the operation is easy and convenient.

Description

technical field [0001] The invention belongs to the technical field of salt tolerance identification of sweet sorghum, and in particular relates to a salt tolerance identification method of sweet sorghum. Background technique [0002] Soil salinization is one of the most serious abiotic stresses in crop production. Currently, at least 20% of the cultivated land in the world is threatened by salinization to varying degrees. my country's existing saline-alkali land is 27 million hm 2 , of which about 7 million hm 2 It is farmland, and its area is still expanding. It is estimated that the direct agricultural losses caused by saline-alkali land in my country amount to tens of billions of yuan every year. With the rapid development of industry, over-exploitation of land, excessive use of chemical fertilizers, unreasonable irrigation and expansion of protected cultivation areas, the area of ​​secondary salinized soil continues to expand, seriously affecting food production and ...

AI Technical Summary

Problems solved by technology

[0004] At present, the main methods for identifying the salt tolerance of sweet sorghum are: sand culture identification method, which can only identify the salt tolerance during seed germination, and as time goes by, the water in the medium is getting less and less, and the salt concentration is getting higher and higher. The higher the value, the shorter the growth time of the seedlings, and gradually wither, the identification results will have large errors and low accuracy; if water is added during the seedling cultivation process, it will affect the consistency of the salt concentration before and after, which will still have a greater impact on the identification results. Statistical analysis; and the test scale should not be too large, it is not suitable for large-scale planting of sweet sorghum screening and variety breeding

The salt tolerance of sweet sorghum seedlings is mainly identified by planting in saline-alkali land. Under natural conditions, the salt distribution in saline-alkali land is uneven, and the results of repeated identification often vary greatly, resulting in large errors in the identification results and low accuracy. Statistical analysis is difficult; and identification Long time, low identification efficiency, heavy workload, waste of manpower and material resources, and low accuracy

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