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Jun date virus-free tissue culture rapid-propagation technology

A technology for tissue culture, rapid propagation and jujube, applied in horticulture, botanical equipment and methods, horticultural tools/equipment, etc., can solve the problems of low reproduction coefficient, easy to spread virus, low yield and so on

Inactive Publication Date: 2015-03-04
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional propagation methods of Junzao mainly include root tiller seedlings and grafted seedlings. Their disadvantages are that they require a lot of propagation materials, have a low reproduction coefficient, cannot be produced every year, and are easy to spread viruses.
Due to the large demand for jujube seedlings, the quality requirements for jujube seedlings are not strict. In many places, there are mixed varieties, serious pests and diseases, and even the possibility of introducing destructive jujube mad seedlings in jujube gardens, resulting in poor quality of jujube. , low yield, low survival rate of long-distance transportation and other unfavorable factors exist

Method used

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  • Jun date virus-free tissue culture rapid-propagation technology
  • Jun date virus-free tissue culture rapid-propagation technology
  • Jun date virus-free tissue culture rapid-propagation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] A kind of jujube detoxification tissue culture rapid propagation technology, mainly comprises following operation treatment process step:

[0173] (1), Junzao jujube stem segment explants start quickly:

[0174] Harvest one-year-old root tiller seedlings in May, take 3 cm jujube stems with jujube axillary buds, clean, sterilize, and treat with hot water at 45°C for 60 minutes to passivate the virus in the material, and put the above-mentioned jujube stems Carry out start-up culture, the culture medium used in the described start-up process is MS+6-BA1.0mg / L+NAA0.2mg / L;

[0175](2), virus-free culture of jujube stem tip:

[0176] Get above-mentioned jujube stem segment after starting culture, peel off the shoot apex meristem of 0.5cm under dissecting microscope and cultivate, and virus-free seedling medium is MS+6-BA1.0mg / L+NAA0.3mg / L; Obtain After the tissue-cultured seedlings were heat-treated at 37°C for 40 days, the stem tips of 0.3 cm were stripped off, and the re...

Embodiment 2

[0182] A kind of jujube detoxification tissue culture rapid propagation technology, mainly comprises following operation treatment process step:

[0183] (1), Junzao jujube stem segment explants start quickly:

[0184] Pick 2-year-old root tiller seedlings in June, take 4 cm jujube stems with jujube axillary buds, clean, sterilize, and after 55 minutes of hot water treatment at 48°C, the virus in the material is inactivated, and the above-mentioned jujube stems Carry out startup culture, the culture medium used in the described startup process is MS+6-BA0.8mg / L+NAA0.15mg / L;

[0185] (2), virus-free culture of jujube stem tip:

[0186] Get above-mentioned jujube stem section after starting culture, peel off the shoot apex meristem of 0.6cm under dissecting microscope and cultivate, and virus-free seedling medium is MS+6-BA0.8mg / L+NAA0.25mg / L; Obtain After the tissue-cultured seedlings were heat-treated at 38°C for 50 days, the shoot tips of 0.1 cm were stripped off, and the r...

Embodiment 3

[0192] A kind of jujube detoxification tissue culture rapid propagation technology, mainly comprises following operation treatment process step:

[0193] (1), Junzao jujube stem segment explants start quickly:

[0194] Harvest 1-year-old root tiller seedlings in June, take 5 cm jujube stems with jujube axillary buds, clean, sterilize, and treat with hot water at 49°C for 50 minutes to passivate the virus in the material, and the above-mentioned jujube stems Carry out start-up culture, the culture medium used in the described start-up process is MS+6-BA0.9mg / L+NAA0.18mg / L;

[0195] (2), virus-free culture of jujube stem tip:

[0196] Get above-mentioned jujube stem section after starting cultivation, peel off the shoot apex meristem of 0.7cm under dissecting microscope and cultivate, and virus-free seedling medium is MS+6-BA0.9mg / L+NAA0.28mg / L; Obtain After the tissue-cultured seedlings were heat-treated at 39°C for 55 days, the shoot tips of 0.1 cm were stripped off, and the...

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Abstract

The invention relates to an agricultural breeding technology and especially relates to a Xinjiang Jun date industrial-seedling virus-free tissue culture rapid-propagation technology. The Xinjiang Jun date virus-free tissue culture rapid-propagation technology mainly comprises the following steps of 1, Jun date tree stem explant rapid-starting, 2, Jun date shoot tip virus-free culture, 3, Jun date virus-free tissue culture seedling propagation and 4, Jun date virus-free tissue culture seedling rooting culture. The Xinjiang Jun date industrial-seedling virus-free tissue culture rapid-propagation technology has a reasonable technology, can be operated simply, is suitable for industrial seedling growing, greatly improves a survival rate, effectively prevents jujube witches' broom disease and is economic and practical.

Description

technical field [0001] The invention relates to agricultural breeding technology, in particular to a technology for virus-free tissue culture and rapid propagation of jujube jujube industrialized seedlings in Xinjiang. Background technique [0002] Xinjiang has a vast territory, abundant water, soil, light and heat resources, and a large temperature difference between day and night. It has unique natural conditions for the development of jujube industry. Jun jujube has high sugar content, high dry matter accumulation, high quality, best-selling fruit, and rising prices. Planting jujube trees has become an important way for Xinjiang's workers and workers to get rich. [0003] Junzao is originally produced in Shanxi, and is now one of the main varieties in Nanjiang and the 14th Division. This variety has strong adaptability, high and stable yield. Large fruit, suitable for drying, good quality, durable for storage and transportation. The traditional propagation methods of Ju...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 付超周雪玲张凡华东来
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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