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Porcine epidemic diarrhea inactivated vaccine and preparation method thereof

A porcine epidemic diarrhea and inactivated vaccine technology, applied in the field of porcine epidemic diarrhea inactivated vaccines and its preparation, can solve the problem of insufficient total secreted antibodies in the intestinal mucosa of susceptible animals, inability to effectively neutralize intestinal viruses, and antibody window long-term issues

Active Publication Date: 2016-08-24
JIANGSU ACAD OF AGRI SCI
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  • Claims
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Problems solved by technology

[0006] The current effective means of prevention and treatment of PED is immunization, but there are problems in the process of prevention and treatment: the antibody production window is long after traditional PED vaccine immunization, which brings great infection risk to susceptible pigs; Infected animals have insufficient intestinal mucosal total secretory antibodies (total intestinal mucosal SIgA) and cannot effectively neutralize intestinal viruses

Method used

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  • Porcine epidemic diarrhea inactivated vaccine and preparation method thereof
  • Porcine epidemic diarrhea inactivated vaccine and preparation method thereof

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Embodiment 1

[0025] 1. Experimental materials

[0026] Monophosphonyl lipid A, muramyl dipeptide, and β-glucan were all purchased from InvivoGen. β-glucosylceramide was purchased from Avanti Polar Lipids, USA. Six-week-old healthy SRC mice were purchased from the Experimental Animal Center of Nanjing Medical University. Epidemic diarrhea antibody detection kit was purchased from Harbin Kehua Biological Company. Mouse intestinal total SIgA ELISA detection kit was purchased from Nanjing Jiancheng Bioengineering Institute. PEDV CV777 Strain: Disclosed in the Study of Porcine Epidemic Diarrhea Inactivated Vaccine Wang Ming, Ma Siqi, etc. China Livestock and Poultry Infectious Diseases 1993 [J].

[0027] 2. Vaccine Preparation

[0028] The preparation method of inactivated vaccine A is as follows:

[0029] (1) Preparation of aqueous phase solution: the inactivated porcine epidemic diarrhea virus liquid (the strain is PEDV CV777 strain, the poison price before inactivation is 5×10 7.5 TCID...

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Abstract

The invention provides a PED (Porcine Epedemic Diarrhea) inactivated vaccine and a preparation method thereof and relates to the field of biopharmacy. The PED inactivated vaccine comprises inactivated PEDV (Porcine Epedemic Diarrhea Virus), and is characterized in that the PED inactivated vaccine comprises 0.05-10 mg / mL Beta-glucosylceramide, 0.1-21 mg / mL monophosphoryl lipid A, 1.5-125 mg / mL muramyl dipeptide and 0.7-4.5 mg / mL Beta-glucan. According to the ingredients of the PED inactivated vaccine, Beta-glucosylceramide, monophosphoryl phosphoryl lipid A, muramyl dipeptide and Beta-glucan have the synergistic effect, the immune response of animals to antigens in the vaccine is significantly improved, the immune window phase is shortened, the antibody production duration of the animal body is obviously prolonged, the serum antibody level is improved, and the level of total intestinal mucosa secretory antibodies (the total SIgA) is improved.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to an inactivated porcine epidemic diarrhea vaccine and a preparation method thereof. Background technique [0002] Porcine Epedemic Diarrhea (PED) is an acute, fecal-oral infectious disease characterized clinically by yellow-green watery diarrhea, vomiting, dehydration, and high mortality in newborn piglets. [0003] The disease was first discovered in the United Kingdom and Belgium in the early 1970s. In 1978, the virus antigen was isolated in my country's Shanghai, Liaoning, Jilin, Heilongjiang and other places. Epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus antigen form, clinical symptoms, and epidemiology are very similar, but there is no cross-reaction between immunology and serology. Currently, the main viral diarrhea in pigs in my country is PED. [0004] The prevalence of PED mainly occurs in winter, but in some areas, pig farms are affected all ye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/215A61P1/12A61K31/716A61K38/05A61K31/661A61K31/7032
Inventor 于晓明陈瑾侯立婷李鹏成乔绪稳吴楠郑其升侯继波
Owner JIANGSU ACAD OF AGRI SCI
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