Tissue culture propagation method of Anemone vitifolia

A technology of wild cotton and tissue culture, which is applied in the field of tissue culture and propagation of wild cotton, can solve problems such as aggravation, contradiction between supply and demand of seedlings, and influence on the application and promotion of wild cotton gardens, and achieve the effect of stable genetic traits

Inactive Publication Date: 2015-03-11
罗翼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, no one has studied the technology of using tissue culture to breed wild cotton, the tissue culture system has not been established, the promotion and popularization of f

Method used

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with the examples, and the following examples are only used to illustrate the present invention, and do not limit the present invention.

[0015] A method for tissue culture propagation of wild cotton, characterized in that the method comprises the following steps:

[0016] (1) Selection of explants: From April to July, the young and tender stems of wild cotton that grow robustly are used as explants;

[0017] (2) Material treatment method: first use a soft brush to gently brush off the dust on the surface of wild cotton, rinse with running water for 1 minute, then irradiate with ultraviolet light for 25 minutes, transfer to the ultra-clean workbench, and use it on the ultra-clean workbench first Soak in 75% ethanol for 20 seconds, then transfer to 0.1% mercuric chloride solution with 3 drops of soil temperature 80 and soak for 1 min, then rinse with sterile water for 3 times, and finally cut the mater...

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Abstract

The invention discloses a tissue culture propagation method of Anemone vitifolia. Excellent effects of the high propagation speed, the stable hereditary character, the high propagation coefficient and vigorous growth of test-tube plantlets are realized mainly through steps of primary culture, propagation culture, rooting culture as well as plantlet exercise and transplantation, the germinating rate of calluses of the Anemone vitifolia is 96%, the propagation multiple is 7.3 times, the rooting rate is 98%, and the final survival rate is 96%.

Description

technical field [0001] The invention relates to a tissue culture propagation method suitable for wild cotton. Background technique [0002] Wild cotton (scientific name: Anemone vitifolia Buch.-Ham.) is a herbaceous plant with a plant height of 60-100 cm. Rhizome oblique, woody, leaf blade heart-shaped or broadly ovate. Scapes are strong, cymes 20-60 cm long, bracts 3, shaped like basal leaves, sepals 5, white or pinkish, obovate, filament-shaped; carpels about 400, aggregate fruit spherical, achenes thin handle. Flowers from July to October. Growing on grassy slopes, ditch sides or sparse forests. Distributed in China, Myanmar, Bhutan, Sikkim, Nepal and India. [0003] At present, no one has studied the technology of using tissue culture to breed wild cotton, the tissue culture system has not been established, the popularization of fine varieties has not been completed, and the contradiction between supply and demand of seedlings is increasing, which seriously affects ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 罗翼
Owner 罗翼
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