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Method for determining content of effective components in injection

A technology of active ingredients and determination methods, applied in the field of content determination of active ingredients in injections, to achieve the effects of good reproducibility, good chromatographic peak symmetry, and no tailing

Inactive Publication Date: 2015-03-25
李志刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the content determination method of active ingredients in Banbianqi 5F injection

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention includes the following steps.

[0014] Weigh the 5F reference substance, add an appropriate amount of methanol to dissolve it, transfer it to a bottle, add methanol to make it up to the mark, and use it as the 5F reference substance stock solution.

[0015] Measure the half-pinner injection and put it into a measuring bottle, add methanol to make the volume up to the mark, and obtain the test solution.

[0016] Put the blank solvent into a measuring bottle, add methanol to make up to the mark, and obtain a negative reference solution.

[0017] Get the reference substance stock solution, the test solution and the negative reference solution, inject samples into HPLC analysis respectively, and record the chromatograms.

[0018] The chromatographic conditions of the HPLC analysis are: the mobile phase is methanol, water, glacial acetic acid; the detection wavelength is 254nm; the flow rate is 1.0mL min -1 ; The column temperature is ...

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Abstract

Belonging to the technical field of content determination, the invention particularly relates to a method for determining the content of effective components in an injection. The method provided by the invention is characterized by good chromatogram display effect, sensitivity and reliability, and good reproducibility. The method provided by the invention comprises the steps of: 1) weighing a 5F reference substance, adding an appropriate amount of methanol to conduct dissolution, then transferring the mixture into a bottle, adding methanol to a constant volume to the scale so as to serve as a 5F reference stock solution; 2) measuring a Pteris semipinnata injection and placing it into a measuring flask, adding methanol to set the constant volume to the scale, thus obtaining a test solution; 3) placing a blank solvent into the measuring flask, and adding methanol to set the constant volume to the scale, thus obtaining a negative reference solution; and 4) taking the reference stock solution, the test solution and the negative reference solution, carrying out sample injection to perform HPLC analysis respectively, and recording the chromatograms.

Description

technical field [0001] The invention belongs to the technical field of content determination, in particular to a method for content determination of active ingredients in injections. Background technique [0002] Banner Banner is a fern of the genus Pteris, which is recorded in the "Chinese Medicine Dictionary" and is widely distributed in southern provinces of my country. It is commonly used in the treatment of snakebite, traumatic bleeding, antibacterial dysentery, enteritis, hepatitis, etc. ?. During the screening of anti-tumor herbal medicines, the research group found that the ethanol and water extracts of Banner Banner showed good anti-tumor activity in vivo and in vitro, and further separated and purified a kaurane diterpene compound. The substance has a rigid tetracyclic diterpene structure, and its antitumor activity may be related to the d, B-unsaturated cyclopentane structure in the molecule. The substance has strong hydrophobicity and is hardly soluble in water...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
Inventor 李志刚尹敏达鲍明煦
Owner 李志刚
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