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A kind of rapid propagation method of passeng vine

A fast and rapid technology of vines, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low seed reproduction rate of vines, susceptibility of cutting seedlings to diseases, long growth cycle, etc., and achieve suitable scale The effects of production, relief of drug pressure on the market, and high rooting rate

Inactive Publication Date: 2016-04-06
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of low seed reproduction rate, long growth cycle, large variability, and easy susceptibility of cutting propagation seedlings of vines, and provide a vines that is suitable for large-scale production, fast propagation, and high survival rate. rapid propagation method

Method used

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  • A kind of rapid propagation method of passeng vine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Selection and treatment of explants

[0024] The explants are derived from the customs clearance rattan plants in the wild in Mengzi County, Yunnan Province. The new shoots of the current year of the customs clearance rattan without pests and diseases are selected, cut into single bud stem segments with one bud as explants, and preliminary sterilization is carried out in the following order: Rinse with running water for 2.5 hours, soak in 5% washing powder water for 5 minutes, and rinse under running water for 25 minutes; soak the preliminarily sterilized cane sections in 75% ethanol solution for 1 minute, and then use mass After soaking in a 0.1% mercuric chloride solution for 12 minutes, wash with sterile water for 3 to 5 times, and dry the surface water of the cane section with sterile paper for use.

[0025] Because the twigs of the customs clearance vine are densely covered with light yellow fluff, many dusts will stick on it, and the general method cannot achi...

Embodiment 2

[0039] Except that the operations of the following steps are different in Embodiment 2, the operations of other steps are the same as those in Embodiment 1, and will not be repeated here.

[0040] (1) Selection and sterilization of explants

[0041] The explants were obtained from the wild vine plants in Mengzi County, Yunnan Province, and were transplanted to the new shoots that grew in the year after domestication in the greenhouse of Yunnan Agricultural University. The other disinfection treatment steps are the same as those of the explant treatment method in Example 1, except that the disinfection time is 11 min with a mass fraction of 0.1% mercuric chloride, and the lowest pollution rate can be achieved.

Embodiment 3

[0043] Except that the operations of the following steps are different in Embodiment 3, the operations of other steps are the same as those in Embodiment 1, and will not be repeated.

[0044] (1) Selection and sterilization of explants

[0045] The explants were derived from the customs clearance vine plants in the wild in Maguan County, Yunnan Province. The new shoots of the customs clearance vine without pests and diseases were selected and cut into single bud stem segments with one bud as the explants. The mass fraction was 0.1% mercury chloride. When the disinfection time was 12min, the pollution rate reached the lowest. Its disinfection time is the same as that of the explants in Example 1 from Mengzi County, Yunnan Province, which is related to the fact that they are all from the wild, and the plants have a lot of dust and germs.

[0046] The test results of Examples 1 to 3 are shown in Table 2.

[0047] Table 2 Test effect of Example 1-Example 3

[0048]

[0049] ...

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Abstract

The invention discloses a rapid marsdenia tenacissima propagation method and belongs to the technical field of vegetative propagation. The method comprises the following steps: selecting fresh tip of marsdenia tenacissima in the same year as an explant, washing by using running water, soaking by using washing powder water, soaking by using ethanol solution, soaking by using 0.1 mass percent of mercury bichloride solution for 11-12 minutes, and performing induction culture, enrichment culture, strong seedling culture and rooting culture. According to the method disclosed by the invention, the induction rate is over 88 percent, the marsdenia tenacissima can be propagated by about 2.5 times per month, the rooting percentage is over 95 percent, the whole propagation period is only 64-72 days, effective and rapid vegetative propagation of the marsdenia tenacissima is realized, and the method is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of vegetative propagation of plants, and in particular relates to a rapid propagation method of Chinese medicinal material vines. Background technique [0002] Clearance vine (Marsdeniatenocissima (Roxb) WightetArm) is a vine of the genus Rhododendron family, also known as black bone vine. It was first published in "Southern Yunnan Materia Medica" and is widely distributed in tropical and subtropical regions of Asia. The whole plant has the effects of clearing away heat and detoxifying, reducing inflammation and pain, relieving cough and relieving asthma. The black bone carcinogen (C 21 - Steroid glycosides and total alkaloids) have a strong destructive effect on the microtubule arrangement, the root of cancer cell division, which can block the mitosis of cancer cells, thereby effectively cutting off the spread and metastasis of cancer cells, and the cancer shrinks until it disappears completely. . It ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 韩俊杨生超颜秋燕候建凯龙光强黄鹤李连文王自林沈雪梅杨丕琼
Owner YUNNAN AGRICULTURAL UNIVERSITY
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