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A kind of preparation method of non-alcoholic fatty liver zebrafish

A non-alcoholic, hepatic zebrafish technology, applied in fish farming, applications, climate change adaptation, etc., can solve the problems of zebrafish death, reduce modeling success rate, operator harm, etc., achieve short maturity period, improve The effect of R&D efficiency and low cost

Active Publication Date: 2017-01-25
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, chemical treatment will cause the death of zebrafish due to its obvious biological toxicity, thereby reducing the success rate of modeling, and it is also harmful to the operator

Method used

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  • A kind of preparation method of non-alcoholic fatty liver zebrafish
  • A kind of preparation method of non-alcoholic fatty liver zebrafish

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Embodiment Construction

[0022] The present invention will be described in further detail below, so that those skilled in the art can implement it with reference to the description.

[0023] A preparation method of non-alcoholic fatty liver zebrafish, comprising the following steps:

[0024] Step 1) Put the sexually mature zebrafish in the mating fish tank according to the ratio of male to female ratio=1:2 under the condition of no light; after 10 hours, put the mating fish tank under the light condition for 0.5 hours to fertilize and lay eggs;

[0025] Step 2) Distribute the fertilized eggs obtained in step 1) in an orifice plate at a density of 30 per well, add 3 to 4 mL of culture solution to each well of the orifice plate, keep the temperature at 28°C, and use 14 hours of light per day / 10 After culturing for 4 dpf in the condition of dark for 4 hours, remove the culture solution in the well plate;

[0026] Step 3) randomly divide the fertilized eggs in the orifice plate into a normal group and a ...

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Abstract

The invention discloses a preparation method for a non-alcoholic fatty liver disease zebra fish. The method comprises the steps that a zebra fish is cultured in high-fat nutrient solution which contains 50-200mu M of palmitic acid and 100-400mu M of oleic acid, and whether the non-alcoholic fatty liver disease zebra fish is successfully constructed or not is judged according to a final liver fat titer of the zebra fish. The preparation method is characterized in that the zebra fish is adopted to construct the non-alcoholic fatty liver disease zebra fish model for the first time in China, cost is low, a mature period is short, and pathology and drug screening cost can be effectively lowered. In addition, a zebra fish model can be used for constructing a high-flux medicine screening platform to further improve biological medicine development efficiency. The achievement of the scheme provides reference for the pathology of the occurrence of the clinical non-alcoholic fatty liver disease, and the formed zebra fish model is provided for various scientific research institutions and pharmaceutical companies, and becomes a powerful tool for the pathological study of the non-alcoholic fatty liver disease, the development of a novel treatment means and the high-flux screening of medicines.

Description

technical field [0001] The invention relates to a method for constructing a disease model animal, in particular to a method for preparing a nonalcoholic fatty liver zebrafish. Background technique [0002] Nonalcoholic fatty liver disease is a clinical condition characterized by steatosis and lipid accumulation in the absence of a history of excessive alcohol consumption. In addition to directly causing liver cirrhosis and liver cancer, the development of the disease also indirectly leads to the occurrence of type 2 diabetes and atherosclerosis. With the prevalence of obesity and related metabolic syndrome, non-alcoholic fatty liver has become an important cause of global chronic liver disease and an important research object of biomedicine. In western developed countries, the incidence of non-alcoholic fatty liver is often between 24% and 42%, while in my country, the incidence of adults has reached more than 15%, and even children have a 2.1% chance of suffering from the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 殷建胡军陈名利白鹏利
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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