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Jujube branches and leaves extract and its preparation method and application

A kind of jujube branches and leaves, extract technology, applied in the direction of drug combination, medical formula, plant raw materials, etc.

Active Publication Date: 2017-09-08
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of pharmacological effects, the sedative, hypnotic and antidepressant effects of Zizyphus spinosus seed are more in-depth, while the reports of anti-insomnia and antidepressant effects of Zizyphus jujube leaves are less

Method used

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  • Jujube branches and leaves extract and its preparation method and application
  • Jujube branches and leaves extract and its preparation method and application
  • Jujube branches and leaves extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] After pulverizing 100 g of jujube branches and leaves, heat and reflux extraction with 20 times the concentration of 80% ethanol solution for 3 times, each time for 1 hour, recover the solvent under reduced pressure, concentrate to obtain the extract, then add distilled water with 10 times the weight of the extract, and ultrasonically Dissolve the extract under the condition of the above solution, pass the above solution through the D101 macroporous resin chromatography column, the amount of macroporous resin is 10 times of the weight of the extract, first wash with water, wash until the phenol-sulfuric acid test solution is negative, and the flow rate is 1 column volume / hour, and then sequentially eluted with ethanol solutions with a volume concentration of 10%, 30%, 40%, 60%, 70% and 90%, the flow rate is 1 column volume / hour, and the dosage is 1 column volume / hour. 10 times the volume, discard the water and 10-30% ethanol eluate to obtain 40%-60% and 70%-90% ethanol e...

Embodiment 2

[0054] After pulverizing 100 g of jujube branches and leaves, use 8 times the concentration of 30% ethanol solution to flash extract twice, each time for 1 min, recycle the solvent, concentrate to obtain the extract, then add distilled water with 5 times the weight of the extract, and extract under ultrasonic conditions Dissolve the extract under water, pass the above solution through D101 macroporous resin chromatography column, the amount of macroporous resin is 10 times the weight of the extract, first wash with water, wash until the phenol-sulfuric acid test solution is negative, and the flow rate is 1 column volume / hour, and then sequentially eluted with ethanol solutions with a volume concentration of 10%, 30%, 40%, 60%, 70% and 80%, the flow rate is 1 column volume / hour, and the dosage is 1% of the column volume 10 times, discard water and 10-30% ethanol eluate, respectively get 40%-60% and 70%-90% ethanol eluate, spectrophotometrically measure the total The content of ...

Embodiment 3

[0056] After pulverizing 100 g of jujube branches and leaves, cold soak and extract 3 times with 10 times the amount of aqueous solution, 3 hours each time, recycle the solvent, concentrate to obtain the extract, then add distilled water with 8 times the weight of the extract, and dissolve the extract under ultrasonic conditions. The above solution is passed through the D101 macroporous resin chromatography column. The amount of the macroporous resin is 10 times the weight of the extract. It is first eluted with water, washed until the reaction of the phenol-sulfuric acid test solution is negative, and the flow rate is 1 column volume / hour, and then sequentially Gradient elution with ethanol solutions with a volume concentration of 10%, 30%, 40%, 60% and 70%, the flow rate is 1 column volume / hour, and the amount used is 10 times the column volume, discarding water and 10% -30% ethanol eluate, get 40%-60% and 70%-90% ethanol eluate respectively, record the total flavonoid conten...

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Abstract

The invention belongs to the technical field of medicine, and relates to extracts of jujube branches and leaves, a preparation method and application thereof, and the application of the extracts of jujube branches and leaves in preparing sedative, hypnotic and antidepressant drugs. The chemical composition of the extract is mainly flavonoids and triterpenoids. The preparation method is to extract the jujube branches and leaves with water or alcohol solvent, concentrate under reduced pressure to obtain the extract, disperse the extract in water, purify it with a macroporous adsorption resin, and combine 40%-60% and 70%-90% ethanol to elute liquid, concentrated under reduced pressure to obtain the ethanol eluate, the total flavonoid content of each part was determined by spectrophotometry to be 45.5%-60.7%, the total triterpene content was 66.7%-73.5%, and the total flavonoid content in the extract obtained by combining the two parts was 15.9% %‑21.1%, the total triterpene content is 43.3%‑47.8%. Using a variety of separation methods, 16 compounds were isolated from the extracts of jujube branches and leaves. Pharmacological tests have proved that the extract of jujube branches and leaves has sedative, hypnotic and antidepressant effects, and can be used in drugs for the treatment of the central nervous system.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine chemistry, and relates to extracts of jujube branches and leaves and a preparation method and application thereof, in particular to extracts of jujube branches and leaves, a preparation method thereof and the application of the extracts in preparing sedative, hypnotic and antidepressant drugs. Background technique [0002] Epidemiological surveys show that about 35.2% of western countries have different degrees of insomnia. A statistical data from the Ministry of Health of China also shows that the number of insomniacs in my country has reached 1.2 million to 1.4 million person-times, and the insomnia rate is as high as 10% to 20%. According to expert estimates, by 2020, there will be more than 700 million insomniacs worldwide. [0003] Long-term insomnia can cause a decrease in the body's immune function and metabolic disorders, and aggravate the original cardiovascular and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/725A61P25/20A61P25/24A61P25/00
Inventor 刘晓秋潘英妮张倩倩路娟
Owner SHENYANG PHARMA UNIVERSITY
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