Liquid culture medium for submerged bamboo fungus fermentation

A liquid culture medium and deep fermentation technology, applied in the field of liquid culture medium for deep fermentation of bamboo fungus, can solve the problems of easy contamination and miscellaneous bacteria, high cost, erosion by diseases and insect pests, etc. Effect

Inactive Publication Date: 2015-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the artificial cultivation period of Dictyophora fruiting body is long and it is easy to pollute miscellaneous bacteria and be eroded by diseases and insect pests. However, mycelium can be quickly obtained through submerged fermentation, which can be used as a liquid strain and extract physiologically active substances from the mycelium.
However, at the present stage, the liquid medium used in submerged fermentation of Dictyophora basically adopts fully synthetic or semi-synthetic medium, and the cost is generally high, which is not conducive to large-scale industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 120g of fresh pig feces, add 500mL of water, fully boil for 25 minutes, stir evenly, filter with 6 layers of gauze, and the obtained filtrate is the pig feces extract;

[0019] Mix 22g of beet molasses, 7g of soybean cake powder, 40mL of pig feces extract, add water to 1000mL, mix well and put them into 500mL triangular flasks, 100mL per bottle, sterilize with high-pressure steam at 121°C for 20min, and cool to obtain the liquid for submerged fermentation of Dictyophora Culture medium;

[0020] Each triangular flask was inoculated with Dictyophora mycelium, the inoculum amount was 5%, the temperature was 28°C, the rotation speed was 100r / min, and the air bath constant temperature shaker was shaken for 12 days to obtain Dictyophora mycelia balls; the Dictyophora mycelium was collected by filtering with 8 layers of gauze The silk ball is freeze-dried to obtain the dry mycelia powder of Dictyophora.

Embodiment 2

[0022] Take 100g of fresh pig feces, add 500mL of water, fully boil for 20 minutes, stir evenly, filter with 6 layers of gauze, and the obtained filtrate is the pig feces extract;

[0023] Mix 20g of beet molasses, 9g of soybean cake powder, 45mL of pig feces extract, add water to 1000mL, mix well and put them into 500mL triangular flasks, each bottle is 100mL, sterilize by high-pressure steam at 121℃ for 25min, and cool to obtain the liquid for submerged fermentation of Dictyophora Culture medium;

[0024] Each triangular flask was inoculated with Dictyophora mycelium, the inoculum amount was 5%, the temperature was 28°C, the rotation speed was 100r / min, and the air bath constant temperature shaker was shaken for 12 days to obtain Dictyophora mycelia balls; the Dictyophora mycelium was collected by filtering with 8 layers of gauze The silk ball is freeze-dried to obtain the dry mycelia powder of Dictyophora.

Embodiment 3

[0026] Take 140g of fresh pig feces, add 500mL of water, fully boil for 30 minutes, stir evenly, filter with 6 layers of gauze, and the obtained filtrate is the pig feces extract; the fresh pig feces are pretreated: freeze the fresh pig feces at -9°C 30min, then placed in a planetary mill for 30min;

[0027] Mix 24g of beet molasses, 5g of soybean cake powder, 50mL of pig feces extract, add water to 1000mL, mix well and put them into 500mL triangular flasks, 100mL per bottle, sterilize with high-pressure steam at 121°C for 30min, and cool to obtain a liquid for submerged fermentation of Dictyophora Culture medium;

[0028] Each triangular flask was inoculated with Dictyophora mycelium, the inoculum amount was 5%, the temperature was 28°C, the rotation speed was 100r / min, and the air bath constant temperature shaker was shaken for 12 days to obtain Dictyophora mycelia balls; the Dictyophora mycelium was collected by filtering with 8 layers of gauze The silk ball is freeze-drie...

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PUM

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Abstract

The invention discloses a liquid culture medium for submerged bamboo fungus fermentation. The liquid culture medium consists of the following components: 20-24g of beet molasses, 5-9g of soybean cake powder, 40-50mL of pig manure extracting solution, and the balance of water which is added to 1000mL. The preparation method comprises the following steps: mixing the various components, performing high-pressure steam sterilization at the temperature of 121 DEG C for 15-25 minutes, and cooling, thereby obtaining the liquid culture medium. The pig manure is matched with the other components so as to prepare the culture medium, the requirements on submerged bamboo fungus fermentation can be met, nutritional ingredients of waste animal residue extracting solution are fully utilized, and the price is low.

Description

technical field [0001] The invention relates to the technical field of bamboo fungus cultivation, in particular to a liquid medium for submerged fermentation of bamboo fungus. Background technique [0002] Dictyophora is a large class of fungi that belong to Eumycotina, Basidiomycotina, Gasteromycetes, Phallales, Phallaceae, Dictyophora. Dictyophora has a certain antiseptic effect and has high nutritional and health value. It is known as the "Queen of Fungi" and is a green food that integrates nutrition, health care and physical therapy. [0003] At present, the fruiting body of Dictyophora spp. has a long period of artificial cultivation and is easy to contaminate miscellaneous bacteria and be eroded by diseases and insect pests. However, mycelium can be quickly obtained through submerged fermentation, which can be used as a liquid strain and extract physiologically active substances from the mycelium. However, at the present stage, the liquid medium used in submerged fer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00
Inventor 邓超付海田尚京迎陈敬华张斯秀黄兴宇徐光宇陈鑫
Owner JIANGNAN UNIV
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