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Cancer urine detection reagent

A technology for detecting reagents and urine, applied in the field of cancer urine detection reagents, can solve problems such as methods and effects that are not stable, and achieve the effects of low cost of reagents, easy promotion and simple operation.

Inactive Publication Date: 2015-05-06
CHONGQING DOUHAO BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the problems in the method between the reaction ion and its dissolved solution, between the reaction ions, etc., as well as the color of the urine sample, the interference of protein and inorganic salts contained in the urine, when the repeated experiments are carried out using the given conditions, its The method and effect are not stable, and the detection reagents need to be further improved in the application of tumor screening and screening to improve the sensitivity and specificity and reduce the missed detection rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] ①. Preparation of solution A: Accurately weigh 2g of iodine, add it to 100ml of distilled water, and after dissolving, set the volume to 250ml;

[0043] ②. Preparation of solution B: Accurately weigh 60g of potassium iodide, add it into 100ml of distilled water, and after dissolving, set the volume to 250ml;

[0044] ③. Preparation of solution C: Accurately weigh 4g of mercuric chloride, add it into 100ml of distilled water, after it dissolves, slowly add 70ml of 12N sulfuric acid, and then add distilled water to 500ml;

[0045] ④. Mix A solution, B solution and C solution in a volume ratio of 100ml:120ml:150ml evenly.

[0046] Detection method: Take 5ml of fresh morning urine after fasting high-protein food and Chinese and Western medicines for 3 days, add 0.5ml of cancer-related factor urine detection reagent, shake well and let stand for 5-15 minutes, observe the presence of precipitation and the presence or absence of precipitation. color.

[0047] Judgment criter...

Embodiment 2

[0049] ①. Preparation of solution A: Accurately weigh 3g of iodine, add it to 100ml of distilled water, and after dissolving, set the volume to 250ml;

[0050] ②. Preparation of solution B: Accurately weigh 60g of potassium iodide, add it into 100ml of distilled water, and after dissolving, set the volume to 250ml;

[0051] ③. Preparation of solution C: Accurately weigh 6g of mercuric chloride, add it into 100ml of distilled water, after it dissolves, slowly add 70ml of 12N sulfuric acid, and then add distilled water to 500ml;

[0052] ④. Mix A solution, B solution and C solution in a volume ratio of 100ml:80ml:120ml evenly.

[0053] Detection method: Take 5ml of fresh morning urine after fasting high-protein food and Chinese and Western medicines for 3 days, add 0.5ml of cancer-related factor urine detection reagent, shake well and let stand for 5-15 minutes, observe the presence of precipitation and the presence or absence of precipitation. color.

[0054] Judgment criteri...

Embodiment 3

[0056] ①. Preparation of solution A: Accurately weigh 3g of iodine, add it to 100ml of distilled water, and after dissolving, set the volume to 250ml;

[0057] ②. Preparation of solution B: Accurately weigh 40g of potassium iodide, add it into 100ml of distilled water, and after dissolving, set the volume to 250ml;

[0058] ③. Preparation of solution C: Accurately weigh 5g of mercuric chloride, add it into 100ml of distilled water, after it dissolves, slowly add 70ml of 12N sulfuric acid, and then add distilled water to 500ml;

[0059] ④. Mix A solution, B solution and C solution in a volume ratio of 100ml:150ml:150ml evenly.

[0060] Detection method: Take 5ml of fresh morning urine after fasting high-protein food and Chinese and Western medicines for 3 days, add 0.5ml of cancer-related factor urine detection reagent, shake well and let stand for 5-15 minutes, observe the presence of precipitation and the presence or absence of precipitation. color.

[0061] Judgment criter...

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Abstract

The invention relates to in-vitro detection reagents for clinical diagnosis and in particular relates to a cancer urine detection reagent. The urine detection reagent is prepared from iodine, potassium iodide, mercury chloride, concentrated sulfuric acid and distilled water. After the test solution is dripped into the urine, the urine immediately develops color, precipitates of different colors are naturally downwards precipitated within 5-15 minutes, whether the detected patients are healthy patients or patients suffering from nephritis, benign tumors and malignant tumors can be judged by virtue of different colors developed by the solution and precipitates. According to the detection reagent disclosed by the invention, the defects in the prior art are overcome, and the detection reagent is a rapid metabolic disorder urine test reagent of a novel formula and has high stability, sensitivity and specificity and wide application range.

Description

technical field [0001] The invention relates to an in vitro detection reagent for clinical diagnosis, in particular to a cancer urine detection reagent. Background technique [0002] In recent years, the incidence of tumor diseases in the world has been on the rise. According to the statistics of cancer worldwide by the American Cancer Society, from 2008 to 2030, the number of new cancers in the world will increase from 1270 per year. million to 21.4 million, and the number of cancer deaths will rise from 7.6 million to 13.2 million. At the same time, my country's statistics in recent years show that it is estimated that in 2020, new cancer cases in China will reach 3.49 million, and the number of deaths from malignant tumors will reach 2.63 million, an increase of 60% compared with 2002. It can be seen that, no matter in developed or developing countries, malignant tumor (cancer) has replaced cardiovascular disease as the disease with the largest number of deaths. Due to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/82
Inventor 宁发子邓小玲罗勇军
Owner CHONGQING DOUHAO BIOTECH
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