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Novel use of 24-acetyl alisol F

A technology of acetylalisitol and doxorubicin, applied in the direction of medical preparations containing active ingredients, organic active ingredients, drug combinations, etc., can solve the problems that the pharmacological activity has not been reported, and achieve the goal of promoting nuclear entry and early apoptosis. death, increased sensitivity

Inactive Publication Date: 2015-06-03
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

24-Acetyl alisitol F is a proterpene-type tetracyclic triterpenoid extracted from Alisma, and its pharmacological activity has not been reported yet

Method used

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  • Novel use of 24-acetyl alisol F
  • Novel use of 24-acetyl alisol F
  • Novel use of 24-acetyl alisol F

Examples

Experimental program
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Effect test

Embodiment 1

[0039] An antitumor pharmaceutical composition is composed of 24-acetyl alisol F and doxorubicin, and the molar concentration ratio of the 24-acetyl alisol F and doxorubicin is 1:1.

[0040] The preparation method of above-mentioned pharmaceutical composition, concrete steps are as follows:

[0041] (1) Take by weighing 24-acetyl alisol F and doxorubicin respectively, for subsequent use;

[0042] (2) Add DMSO (dimethyl sulfoxide) solvent to dissolve 24-acetyl alisol F, and obtain 24-acetyl alisol F mother liquor;

[0043] (3) add ultrapure water to dissolve adriamycin to obtain a doxorubicin mother liquor;

[0044] (4) Take RPMI1640 basal culture, dilute the 24-acetyl alisol F mother liquor step by step to obtain 24-acetyl alisitol F solutions with molar concentrations of 5, 10, and 20 μM;

[0045] (5) Take RPMI1640 basal culture, dilute the doxorubicin mother liquor step by step to obtain a doxorubicin solution with a molar concentration of 10 μM;

[0046] (6) Take 24-acet...

Embodiment 2

[0048] An antitumor pharmaceutical composition is composed of 24-acetyl alisol F and doxorubicin, and the molar concentration ratio of the 24-acetyl alisol F and doxorubicin is 0.5:1.

[0049]The preparation method of above-mentioned pharmaceutical composition, concrete steps are as follows:

[0050] (1) Take by weighing 24-acetyl alisol F and doxorubicin respectively, for subsequent use;

[0051] (2) Add DMSO (dimethyl sulfoxide) solvent to dissolve 24-acetyl alisol F, and obtain 24-acetyl alisol F mother liquor;

[0052] (3) add ultrapure water to dissolve adriamycin to obtain a doxorubicin mother liquor;

[0053] (4) Take RPMI1640 basal culture, dilute the 24-acetyl alisol F mother liquor step by step to obtain 24-acetyl alisitol F solutions with molar concentrations of 5, 10, and 20 μM;

[0054] (5) Take RPMI1640 basal culture, dilute the doxorubicin mother liquor step by step to obtain a doxorubicin solution with a molar concentration of 10 μM;

[0055] (6) Take 24-ace...

Embodiment 3

[0057] An antitumor pharmaceutical composition is composed of 24-acetyl alisol F and doxorubicin, and the molar concentration ratio of the 24-acetyl alisol F and doxorubicin is 2:1.

[0058] The preparation method of above-mentioned pharmaceutical composition, concrete steps are as follows:

[0059] (1) Take by weighing 24-acetyl alisol F and doxorubicin respectively, for subsequent use;

[0060] (2) Add DMSO (dimethyl sulfoxide) solvent to dissolve 24-acetyl alisol F, and obtain 24-acetyl alisol F mother liquor;

[0061] (3) add ultrapure water to dissolve adriamycin to obtain a doxorubicin mother liquor;

[0062] (4) Take RPMI1640 basal culture, dilute the 24-acetyl alisol F mother liquor step by step to obtain 24-acetyl alisitol F solutions with molar concentrations of 5, 10, and 20 μM;

[0063] (5) Take RPMI1640 basal culture, dilute the doxorubicin mother liquor step by step to obtain a doxorubicin solution with a molar concentration of 10 μM;

[0064] (6) Take 24-acet...

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Abstract

The invention provides novel use of 24-acetyl alisol F (ALI). The 24-acetyl alisol F can be used for increasing the cumulant of adriamycin (DOX) in cells to promote adriamycin to enter into nucleus so as to remarkably promote early apoptosis of an MCF-7 / ADR cell (human breast cancer-adriamycin-resistant cells). The 24-acetyl alisol F has an important medical development value in preparing anti-tumor drugs. Due to the action of the 24-acetyl alisol F, the composition prepared from the 24-acetyl alisol F and adriamycin can be used for effectively increasing the sensitivity of the anticancer drug adriamycin and further reducing the side effects of existing chemotherapeutical drugs while improving the chemotherapeutical effect.

Description

technical field [0001] The present invention relates to the new application of proterpene type tetracyclic triterpenes, especially the new application of 24-acetyl alisol F. Background technique [0002] Chemotherapy is a main means of tumor treatment, but the phenomenon of drug resistance of tumor cells is unavoidable, which is the main obstacle of tumor chemotherapy, and also the main reason for the poor effect or even failure of chemotherapy. [0003] Clinically, many chemotherapeutic drugs can achieve good results in the initial treatment of tumors, but they will relapse after a period of time, and the therapeutic effect after recurrence is poor. The main reason is that tumor cells develop resistance to chemotherapeutic drugs. It is estimated that drug resistance exists in more than 90% of cancer deaths. Therefore, the study of tumor drug resistance is of equal importance to the study of treatment methods. [0004] There are many factors that lead to drug resistance of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P35/00A61K31/58
Inventor 潘桂湘王晓明朱彦曾青青刘二伟李婷婷
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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