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A method for biological control of cistanche for disease resistance and growth promotion

A technology of Cistanche deserticola and biological fertilizer, which is applied to the field of biological control, disease resistance and growth promotion of Cistanche deserticola, can solve the problems of poor resistance, low stress resistance of spore-free bacteria, low stability and the like, and achieves simple treatment, good economic benefits, and no waste water. The effect of emissions

Inactive Publication Date: 2017-07-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, non-spore bacteria have low stress resistance and poor resistance to dry high temperature, so the stability is not high when made into liquid or adsorbent

Method used

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  • A method for biological control of cistanche for disease resistance and growth promotion
  • A method for biological control of cistanche for disease resistance and growth promotion
  • A method for biological control of cistanche for disease resistance and growth promotion

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.33×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated 2%, shake culture at 37°C for 24 hours, and the density of the bacterial solution is 2.48×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0028] 2) Cut a total of 100kg of healthy branches of Cistanche into small sections for natural composting, spray 2L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, continue composting, and compost when the internal temperature of the compost exceeds 43°C Finished, stirred and cooled, post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing Bacillus subtilis, that is, a bio-fertili...

Embodiment 2

[0038] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.75×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated The amount is 3%, shake culture at 37°C for 24 hours, and the density of the bacterial solution is 3.15×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0039] 2) Cut a total of 100kg of healthy branches of Cistanche into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, continue composting, and compost when the internal temperature of the compost exceeds 43°C At the end, stir and let it cool, post-ferment in a natural state, and become a bio-fertilizer rich in beta-glucanase-producing Bacillus subtilis.

[...

Embodiment 3

[0045] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.68×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated The amount is 3%, cultured with shaking at 37°C for 24 hours, and the density of the bacterial solution is 2.79×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0046] 2) Cut a total of 100kg of healthy branches of Cistanche into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, continue composting, and compost when the internal temperature of the compost exceeds 43°C At the end, stir and let it cool, post-ferment in a natural state, and become a bio-fertilizer rich in beta-glucanase-producing Bacillus subtil...

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Abstract

The invention discloses a method for biologically controlling and resisting disease and promoting growth of cistanche. The method comprises the following steps: preparing bacillus subtilis as a high-density bacterium suspension in density of 109-1010cfu / mL, scissoring health branches of the cistanche into small sections to compost, spraying bacillus subtilis high-density bacterium suspension in the composting process, fermenting to obtain bio-fertilizer capable of effectively inhibiting and killing pathological fungus in the cistanche, wherein the bio-fertilizer is used for biologically controlling and resisting disease and promoting growth of the cistanche. According to the invention, the bacillus subtilis for producing beta-glucanase is plant probiotics for biologically controlling and resisting disease and promoting growth of the cistanche, the biological control and growth promoting effect on the pathological fungus of the cistanche are obviously enhanced after the bacillus subtilis is added in the composting; the compositing process treatment is simple and convenient, free from wastewater discharge; the method is less in investment, low in energy consumption, easy to operate, easy for industrial large-scale production, and has good economic benefit and wide application prospect.

Description

technical field [0001] The invention relates to the field of preparation of biological control fertilizers, in particular to a method for biological control of Cistanche deserticola for disease resistance and growth promotion. Background technique [0002] Cistanche deserticola (scientific name: Cistanche deserticola Ma) is a tall herb, 40-160 cm high, mostly underground, with flowering period from May to June and fruit period from June to August. [0003] Cistanche is a parasitic plant that lives on the roots of the desert tree Haloxylon, absorbing nutrients and water from the Haloxylon host. Known as "Desert Ginseng", it has extremely high medicinal value and is a traditional Chinese medicinal material. Cistanche is sweet in taste and warm in nature. It has the functions of nourishing kidney and strengthening yang, nourishing essence and marrow, nourishing blood and moistening dryness, pleasing color and prolonging life. Cistanche has both medicinal and edible uses. Long...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F17/00
CPCC05F11/00C05G3/00C05F11/08C05F17/00Y02W30/40
Inventor 胡慧雯徐薇薇吴渊阮晖杜岩松许丽珍吴孔华莫建平史金昕波缪丹俞雨吴盛彬徐腾洋
Owner ZHEJIANG UNIV
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