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Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons

A technology of Pantoea pineapple and root growth, applied in the field of microorganisms, can solve the problems of phosphorus deficiency in cultivated land soil, etc., and achieve the effect of improving root system composition, growth promotion, and enhanced ability

Active Publication Date: 2015-06-10
QIQIHAR UNIVERSITY +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Phosphorus is one of the macronutrient elements necessary for plant growth, but there is more phosphorus in the soil than Ca 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate, only a small part can be absorbed and utilized by plants, which leads to the widespread phenomenon of phosphorus deficiency in cultivated soil (Taurian T, Anzuay M S, Angelini J G, et al. Phosphate-solubilizing peanut associated bacteria: screening for plant growth -promoting activities[J]. Plant and soil, 2010, 329(1-2): 421-431.)

Method used

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  • Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons
  • Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons
  • Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons

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Embodiment 1

[0027] Example 1 Isolation and identification of Pantoea ananatis HYL01 (Pantoea ananatis HYL01) strain and its application in promoting the growth of watermelon roots

[0028] 1 Materials and methods

[0029] 1.1 Culture medium

[0030] PKO inorganic medium (1L): glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.036g, MnSO 4 ·H 2 O 0.03g, Ca 3 (PO 4 ) 3 2.0g, agar 20g, distilled water to 1000ml, liquid medium without agar.

[0031] 1.2 Purification and screening of phosphorus-dissolving strains

[0032] The plant rhizosphere soil of gramineous crops in Qiqihar City, Heilongjiang Province was collected in March 2014, and the root surface soil was collected by shaking soil method, put into sterile bags, and stored in a refrigerator at 4°C for later use. Weigh 1g of root mixed soil respectively, put it into a 250ml Erlenmeyer flask containing 9ml sterile water and 8 sterile glass beads according to aseptic operation, pla...

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Abstract

The invention discloses a separated pantoea ananatis HYL01 strain and an application thereof in promotion of root growth of watermelons. The separated pantoea ananatis HYL01 strain is named pantoea ananatis HYL01 which is preserved in China Center for Type Culture Collection in Wuhan University on October 29, 2014, and preservation No. is CCTCC NO.M 2014530. Research results show that the strain HYL01 is relatively strong in phosphorus dissolving capacity, and effective dose of dissolving Ca3(PO4)3 is 140.26mg / L; meanwhile, the strain HYL01 has a capacity of secreting IAA and the maximum secretion quantity is 11.39mg / L. An HYL01 fungicide, after being applied, can significantly promote the increase in total root length, root surface area, root volume and specific root length of watermelon seedlings, and can remarkably increase the proportion of watermelon seedling hairy root length that root diameter is more than 0 and less than or identical to 0.5mm; therefore, the separated pantoea ananatis HYL01 strain disclosed by the invention has a potential of preparing a compound microorganism bacterium fertilizer capable of promoting the root growth of watermelons.

Description

technical field [0001] The invention relates to a phosphorus-dissolving bacteria, in particular to a Pantoea ananatis with auxin (IAA) synthesis ability, and also to the application of the strain in promoting the growth of watermelon roots. The invention belongs to the technical field of microorganisms. Background technique [0002] Phosphorus is one of the macronutrients necessary for plant growth, but more phosphorus in soil is associated with Ca 2+ , Fe 3+ , Fe 2+ , Al 3+ Combined to form insoluble phosphate, only a small part can be absorbed and utilized by plants, which leads to the widespread phenomenon of phosphorus deficiency in cultivated soil (Taurian T, Anzuay M S, Angelini J G, et al. Phosphate-solubilizing peanut associated bacteria: screening for plant growth -promoting activities[J]. Plant and soil, 2010, 329(1-2): 421-431.). Soil phosphorus-dissolving microorganisms can convert fixed phosphorus that cannot be absorbed and utilized by plants into absorbab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 王志刚徐伟慧李珊珊胡云龙胡影刘帅王春龙
Owner QIQIHAR UNIVERSITY
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