Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons
A technology of Pantoea pineapple and root growth, applied in the field of microorganisms, can solve the problems of phosphorus deficiency in cultivated land soil, etc., and achieve the effect of improving root system composition, growth promotion, and enhanced ability
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[0027] Example 1 Isolation and identification of Pantoea ananatis HYL01 (Pantoea ananatis HYL01) strain and its application in promoting the growth of watermelon roots
[0028] 1 Materials and methods
[0029] 1.1 Culture medium
[0030] PKO inorganic medium (1L): glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.036g, MnSO 4 ·H 2 O 0.03g, Ca 3 (PO 4 ) 3 2.0g, agar 20g, distilled water to 1000ml, liquid medium without agar.
[0031] 1.2 Purification and screening of phosphorus-dissolving strains
[0032] The plant rhizosphere soil of gramineous crops in Qiqihar City, Heilongjiang Province was collected in March 2014, and the root surface soil was collected by shaking soil method, put into sterile bags, and stored in a refrigerator at 4°C for later use. Weigh 1g of root mixed soil respectively, put it into a 250ml Erlenmeyer flask containing 9ml sterile water and 8 sterile glass beads according to aseptic operation, pla...
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