Anticoagulant, preparing method of anticoagulant and method of preparing blood smear with same

Abstract

The invention provides anticoagulant, a preparing method of anticoagulant and a method of preparing a blood smear with the same. The preparing method includes: measuring heparin sodium and distilled water, and fully dissolving the heparin sodium in the distilled water to obtain anticoagulant mother liquor; diluting the anticoagulant mother liquor with a low-volume syringe to obtain anticoagulant diluent to be dried; quantitatively injecting the anticoagulant diluent to be dried into a high-volume syringe with the low-volume syringe; drying the high-volume syringe holding the anticoagulant diluent to be dried, at 40 DEG C to 60 DEG C. The method of preparing the blood smear with the anticoagulant includes: taking a blood sample with the high-volume syringe holding the anticoagulant; mixing the blood sample taken with the high-volume syringe; preparing the blood smear with a slide through the blood obtained in the step f. The anticoagulant, the preparing method of the anticoagulant and the method of preparing the blood smear with the same have the advantages that unexperienced students are enabled to acquire the clear blood smear and the morphological structure and features of blood cells are truly reproduced.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method of an anticoagulant, an anticoagulant and a method for preparing a blood smear using the anticoagulant. Background technique [0002] The production of blood smears is widely used clinically. The preparation of blood smears with suitable thickness, uniform distribution and good staining is one of the important basic techniques of hematological examination. [0003] In the production of blood smears, pushing the smear is the difficulty and key in the whole production technology. In the current undergraduate teaching experiment, there is an experiment of making and observing blood smears. At present, the main method used in the laboratory is to directly collect blood and directly make blood smears, but for the vast majority of students, it is the first time for them to push the slides, without the slightest experience, they often drop blood on the slides , before p...

AI Technical Summary

Problems solved by technology

At present, the main method used in the laboratory is to directly collect blood and directly make blood smears, but for the vast majority of students, it is the first time for them to push the slides, without the slightest experience, they often drop blood on the slides , it is too late to push the slide, the blood has coagulated on the slide, so it is necessary to use the blood added with a small amount of anticoagulant to make the blood smear

[0004] In actual operation, the exact dose of anticoagulant is often difficult to grasp with less blood volume. In actual operation, due to the accuracy of anticoagulant dose many times, red blood cells in the blood smears made Severely deformed, which led to the complete failure of the entire experiment

This is because the amount of blood used to make a blood smear is very small, and the amount of anticoagulant added is also required to be very small. In this case, the amount added is required to be very precise. Excessive amount will damage the cells. The shape of red blood cells, especially red blood cells, will be severely deformed and jagged; if the dosage is too small, the anticoagulant effect will not be achieved

However, if such a small precise dosage is to be directly used and directly added, it needs to be measured by a very precise instrument, which is expensive; if the conventional method is used for dissolution and constant volume, it is not only time-consuming, but also requires abundant operating experience

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