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Boletus aereus stock culture low-temperature preservation method

A technology for cryopreservation of black boletus, which is applied in the field of cryopreservation of the mother species of black boletus, can solve the problems of insufficient thick and sturdy mycelium, difficult to obtain, consumption of nutrients, and the like, and achieves that the cryopreservation method is simple, effective and solves the problem. The effect of simple species preservation and high application value

Active Publication Date: 2015-07-08
贵州宏臻菌业投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if directly stored at a constant temperature of 10°C to 15°C, the nutrients of the bacteria will be seriously consumed. After one year of preservation, the bacteria will be activated, the vitality of the bacteria will be significantly reduced, the mycelia will not be dense and strong, and the growth will be slow. Light weight, lower quality; and the solid medium has more components, especially related to red loam, and the composition of red loam varies greatly, and it is not easy to obtain

Method used

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  • Boletus aereus stock culture low-temperature preservation method
  • Boletus aereus stock culture low-temperature preservation method
  • Boletus aereus stock culture low-temperature preservation method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Cultivation of black boletus parent species: collect wild boletus fruiting bodies, use tissue separation method to inoculate tissue pieces of black boletus in solid medium, culture at a constant temperature of 28°C, and mycelia will be overgrown in 25 days culture medium, to obtain the female species of Boletus nigricans;

[0034] Wherein, the formula of solid medium is as shown in table 1, and the mycelia block that different solid medium is cultivated is directly cultivated simultaneously, obtains black boletus fruiting body, and black boletus fruiting body has measured weight, specifically as table 1.

[0035] Table 1 Solid medium with different content components

[0036]

[0037] As can be seen from Table 1, the mycelium block of the black boletus that the solid medium of different component contents cultivates is cultivated, and the fruiting body of the black boletus that obtains is heavy, illustrates that the solid medium of different component contents c...

Embodiment 2

[0054] (1) Cultivation of black boletus parent species: collect wild boletus fruiting bodies, use tissue separation method to inoculate tissue pieces of black boletus in solid medium, culture at a constant temperature of 26°C, and mycelia will be overgrown in 28 days Substratum, obtains the boletus female species, wherein, solid medium adopts formula 1 in the embodiment 1 to make;

[0055] (2) Preservation and storage tube of female species is made: with a test tube of 18 × 180mm specification, 15ml of distilled water is adorned in each test tube, and then routinely sterilized for later use; Cut 0.5㎝×0.5㎝ mycelium pieces and put them in distilled water in sterilized test tubes, put 5 pieces in each test tube, plug the test tube mouth with a silica gel stopper, and get the stock storage tube;

[0056] (3) Pre-cooling: the parent seed storage tube obtained in step (2) is pre-cooled for 30 days in a thermostat at 15°C;

[0057] (4) Low-temperature preservation: put the slanted m...

Embodiment 3

[0065] (1) Cultivation of black boletus parent species: collect wild black boletus fruiting bodies, use tissue separation method to take black boletus tissue pieces and inoculate them in solid medium, culture at a constant temperature of 30°C, and the mycelia will be overgrown in 22 days Substratum, obtains the boletus female species, wherein, solid medium adopts formula 1 in the embodiment 1 to make;

[0066] (2) Preservation and storage tube of female species is made: with a test tube of 18 × 180mm specification, 12ml of distilled water is adorned in each test tube, and then routinely sterilized for later use; Cut 1cm×1cm mycelium pieces and put them in distilled water in sterilized test tubes, put 3 pieces in each test tube, plug the test tube mouth with a silica gel stopper, and get the stock storage tube;

[0067] (3) Pre-cooling: the parent seed storage tube obtained in step (2) is pre-cooled in a thermostat at 12°C for 20 days;

[0068] (4) Low-temperature preservation...

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Abstract

The invention relates to the technical field of culture preservation of edible fungus cultivation and especially relates to a boletus aereus stock culture low-temperature preservation method which includes following steps: (1) cultivating mycelia of boletus aereus fruiting body on a solid culture medium to obtain a mycelia block; (2) soaking the mycelia block in sterilized distilled water in a sterilization container, sealing the container, and allowing the mycelia block to stand for 10-30 days at 10-15 DEG C; and (3) performing low-temperature preservation at 3.5-4.5 DEG C. According to the method, the stock culture is preserved in a step-by-step refrigerating manner. A test proves that the stock culture preserved through the method is equal to the first generation stock culture in mycelia activity and bacterial strain cultivation characters. The method can keep the stock culture activity and excellent characters of the boletus aereus, can solve the problem of middle- and short-period simple bacterial strain preservation of the stock culture, and can provide a powerful guarantee of large-scale cultivation.

Description

technical field [0001] The invention relates to the field of culture preservation of edible fungi, in particular to a method for low-temperature preservation of boletus female species. Background technique [0002] Black boletus (Phlebopus portentosu) is a precious and most popular edible fungus in tropical areas, distributed in Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, New Zealand and my country's Yunnan, Guangxi and Hainan . Black boletus is fresh and tender in quality, delicious in taste, rich in nutritional value, high in protein and low in fat, rich in 17 kinds of essential amino acids and mineral elements necessary for human body such as phosphorus, potassium, calcium, magnesium, iron and zinc. Good health food and has good medicinal value, deeply loved by consumers. At present, the method of cultivating saprophytic bacteria can be used for artificial cultivation, and host trees such as poinciana, coffee, pomelo, and mango host trees can be i...

Claims

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Application Information

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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 纪光燕周茂纪光玉杨太茂杨君巧谢飞
Owner 贵州宏臻菌业投资发展有限公司
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