Method for noninvasive production of chronic obstructive pulmonary disease acute exacerbation animal model

Abstract

The invention discloses a method for noninvasive production of a chronic obstructive pulmonary disease acute exacerbation animal model, a bacterium solution instilling manner of endoscope-guided intratracheal instillation is used, the equipment are as follows: a nose 0 degree 4 # hard endoscope is used, a xenon lamp cold light source is used, and a panasonic KS-822 videography surveillance system is used; a rat is placed in a dryer with the diameter of 26 cm for ether anesthesia, after satisfied anesthesia, the rat is rapidly taken from an anesthetic machine, and placed in a supine position, limbs of the rat are respectively fixed on a rat board with elastic fixing clamps, 6 u (0.15 ml) of elastase solution (7.7 mu / mg, Sigma company) is slowly instilled, 100 mu l of air is then filled, at the moment, the rat is in inspiratory phase, drugs enter into bronchia along with air flow during inspiration, the rat recovery time is 30-60 seconds, and then the rat is placed in a constant temperature animal room for breeding; after inoculation, the general situations of activity, food taking, spirit of the rat are daily observed, and corresponding data is recorded.

Description

Technical field [0001] The invention belongs to the technical field of animal experimental model construction, and specifically relates to a method for non-invasively making an animal model of acute exacerbation of chronic obstructive pulmonary disease. Background technique [0002] At present, there are many methods for constructing animal models of acute exacerbation of chronic obstructive pulmonary disease. The longest used methods include: endotoxin injecting into the trachea and smoking cigarettes, instilling protease into the trachea and sulphur dioxide stimulation method, persistent bacterial infection and smoking Method, inject elastase and lipopolysaccharide into the airway, inject protease into the airway plus normal pressure hypoxia method, CaCl 2 Combine Shanli bean preparations. All of the above modeling methods require instillation of chemical agents into the airways. Since the establishment of acute exacerbation of chronic obstructive pulmonary disease animal model...

AI Technical Summary

Problems solved by technology

At this time, the root of the tongue is pulled to expose the glottis, but the head of the rat is long and narrow, and the glottis is narrow and deep in the oral cavity. , it will block most of the line of sight. During the short time when the glottis is exposed, it is difficult to accurately insert the glottis to complete the operation, and it is easy to mistake the drug delivery device into the esophagus or insert it repeatedly due to unclear vision, resulting in laryngeal edema and airway edema. Injury, vocal cord hemorrhage, even death of rats in severe cases

[0005] Blind insertion method: intratracheal administration through the mouth, without exposing the glottis, it is easy to repeatedly stimulate the larynx, cause mucosal edema and bleeding, increase airway secretions, and cause airway obstruction. In addition, it is very easy to mistake the injection device into the esophagus , with a low success rate

For percutaneous intratracheal drug administration, it is necessary to directly and accurately locate the tracheal position from the external skin and administer the drug from the cricoid cartilage space, which requires high technical requirements for the operator, otherwise it is easy to stimulate local muscles and fascia many times, and the drug effect is difficult to evaluate , It is also easy to cause edema and bleeding, and the success rate is low

Images