Use of miRNA-Cyfra21-1 combination in detection of non-small cell lung cancer

A non-small cell lung cancer, expression level technology, applied in the field of biomedicine, can solve the problems of questionable stability, great influence on the diagnostic efficiency of circulating miRNA, and complicated operation process.

Active Publication Date: 2015-08-05
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

McDonald et al. found that some differences in pre-analysis and data analysis methods have a great impact on the diagnostic efficiency of circulating miRNAs
They found that different RNA extraction methods were the main reason for the internal imprecision of the experiment
Because miRNAs are shorter and less abundant in blood, the stability of miRNA extraction by column adsorption is questionable
In addition, although the accuracy of the traditional Trizol extraction method is better than that of the column adsorption method, its operation process is relatively complicated

Method used

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  • Use of miRNA-Cyfra21-1 combination in detection of non-small cell lung cancer
  • Use of miRNA-Cyfra21-1 combination in detection of non-small cell lung cancer
  • Use of miRNA-Cyfra21-1 combination in detection of non-small cell lung cancer

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Embodiment 1

[0071] Example 1. Combined detection of non-small cell lung cancer by miRNA and Cyfra21-1

[0072] The experimental design diagram of the present invention is as follows figure 1 shown.

[0073] 1. Specimen collection and clinical information

[0074] From November 2009 to July 2010, serum samples from 44 cases of non-small cell lung cancer (22 cases of lung adenocarcinoma and 22 cases of lung squamous cell carcinoma) and 22 age- and sex-matched normal serum samples (as control samples) were collected For screening work, this group is a discovery group, which is used to screen abnormally expressed miRNAs from 380 candidate miRNAs.

[0075] From February to July 2013, 178 cases of non-small cell lung cancer (105 cases of lung squamous cell carcinoma and 73 cases of lung adenocarcinoma) serum samples and 122 cases of normal serum samples (as control samples) were randomly divided into training group (91 cases of carcinoma, 61 cases of normal) and verification group (87 cases ...

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Abstract

The invention discloses a use of miRNA-Cyfra21-1 combination in detection of non-small cell lung cancer. The invention discloses a use of substances for detecting miR-652, miR-660 and Cyfra21-1 expression levels in preparation of products for screening and / or auxiliary diagnosis of non-small cell lung cancer. The combination of miR-652, miR-660 and Cyfra21-1 has the optimal non-small cell lung cancer detection efficiency superior to those of two miRNA combination and single Cyfra21-1 (P of less than 0.01). The disclosed composition has an important use in non-small cell lung cancer detection.

Description

technical field [0001] The invention relates to the combined application of miRNA and Cyfra21-1 in the detection of non-small cell lung cancer, which belongs to the field of biomedicine. Background technique [0002] Lung cancer is the malignant tumor with the highest morbidity and mortality in the world. About 80% of lung cancer is non-small cell lung cancer (NSCLC), which is mainly divided into two subtypes: lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SCC). So far, the most effective way to treat non-small cell lung cancer is surgical treatment. However, patients usually have no obvious symptoms in the early stage, which makes some patients lose the best opportunity for treatment. Currently, the commonly used diagnostic methods for lung cancer include chest X-ray, CT and other imaging methods. However, both low-dose helical CT and chest X-rays have a high false positive rate. In addition, low-dose spiral CT also has two potential risks of overdiagnosis an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/574
Inventor 赫捷陈照丽周成诚
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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