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Method for determining gastrodine content in gastrodia elata

A determination method and technology for gastrodin, which is applied in the field of chemical detection, can solve the problems of long test time, high technical requirements of testers, large amount of solvent, etc., and achieve the effect of less amount of solvent, clear detection accuracy, and high measurement accuracy.

Active Publication Date: 2015-08-05
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses a large amount of solvent, takes a long time to test, and requires high skills of testers

Method used

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  • Method for determining gastrodine content in gastrodia elata
  • Method for determining gastrodine content in gastrodia elata
  • Method for determining gastrodine content in gastrodia elata

Examples

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Embodiment 1

[0025] The instruments and consumables used in this embodiment are: Pulverizer, Electronic Analytical Balance (XA205DU): Sensitivity 0.001g, Accelerated Solvent Extractor Model: ASE-350 (10ml extraction pool, 50ml collection bottle), volumetric bottle: 25ml, Centrifuge tube: 2ml.

[0026] Step 1: Take about 1g of Gastrodia elata powder (passed through a No. 3 sieve), weigh it accurately, mix it with 1g of diatomaceous earth, and set aside;

[0027] Step 2: Pad 3 to 6 sheets (1 piece of commercial filter paper) at the bottom of the extraction pool with homemade circular filter paper (diameter 27mm), add about 1g of diatomaceous earth, then add the homogeneously mixed sample obtained in step 1, and then add an appropriate amount of diatomaceous earth , shake gently to make it at the same level as the pool mouth, and tighten the upper cover of the extraction pool.

[0028] Step 3: extract and extract with ASE to obtain the extract, the specific parameters are shown in Table 1 be...

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Abstract

The invention discloses a method for determining gastrodine content in gastrodia elata, which comprises the following steps: 1. pulverizing gastrodia elata, and mixing with diatomite, wherein the weight ratio of the gastrodia elata to the diatomite is 1:1, and the pulverized gastrodia elata is the gastrodia elata powder screened through a 50-mesh sieve; 2. extracting gastrodine by an ASE (accelerated solvent extraction) extraction process, and collecting the extracting solution, wherein the extraction solvent adopted by the ASE extraction process is a 49.5-50.5 vol% dilute ethanol solution; 3. drying the extracting solution by distillation, dissolving in an acetonitrile-0.05% phosphoric acid water solution mixture, diluting to the specific volume, centrifugating, and taking the supernate; and 4. determining the gastrodine content in the supernate by high-performance liquid chromatography. The invention aims to provide a method for determining gastrodine content in gastrodia elata, which has the advantages of high extraction speed and high measurement accuracy.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a method for determining the content of gastrodin in Gastrodia elata. Background technique [0002] Gastrodia elata is the dry tuber of the orchid plant Gastrodiaelata B1. It is excavated from the beginning of winter to before Qingming of the next year, washed immediately, steamed thoroughly, and dried at low temperature in the open. Gastrodia elata is oval or strip-shaped, slightly flat, shrunken and slightly curved, 3-15cm long, 1.5-6cm wide, and 0.5-2cm thick. The surface is yellowish-white to light yellowish-brown, with longitudinal wrinkles and multiple rounds of horizontal rings arranged by latent buds, and sometimes tan fungal cords can be seen. There are reddish-brown to dark brown parrot-shaped buds or residual stem bases at the top; there is a round navel-shaped scar at the other end. The texture is hard, not easy to break, the section is relatively flat, ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 陈学松陈翠玲
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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